The Study On The Effects Of Physical And Chemical Factors On Flaonvoid Production In Tissue Culture Of Ginkgo Biloba L.

This article chose callus induced by young leave of Ginkgo biloba L. as the materials, and after successive transfer culture, the loose cell groups are selected for liquid culture. The effects of different physical, chemical and biotic factors on flavonoid content in callus of Ginkgo biloba L. are checked. The various concentrations of La3+ are added to media to culture the Callus of Ginkgo biloba L. The growth ratios of callus containing La3+ of 10-4 g/L, 10-3g/L, 10-2g/L, 10-1g/L are superior to that of control, and 10-4g/L is the most appropriate concentration. The similar results have been gained in the subculture and liquid culture. There are obvious results that concentrations of La3+ have the adverse correlation with flavonoid production. When organisms are in adversity, concentration of Ca2+ in organisms will increase and enzymes involved in flavonoid production need Ca2+. So the further study is going on of various concentrations of Ca2+ on flavonoid production. The solid medium cultivating callus are added to various concentrations of Ca2+, while the results of flavonoid production and concentrations of Ca2+ needs profound research. After the callus were cultivated in the basic medium lacking Ca2+ for half a year, the various concentrations of La3+ are added to media. The flavonoid content of callus in media containing La3+ is less than that of control, and the effects of different concentrations of La3+ on flavonoid content are slight. The flavonoid content in callus is less and less, with the length of time of successive transfer culture. After Subambient temperature(4~6℃) treating callus 30mins every day, the flavonoid content varies slightly. Then after Subambient temperature(4~6℃) treating callus 30mins, flavonoid content is determined at once. The content is higher than that in the callus under room temperature for 30mins after Subambient temperature treating. And Subambient temperature(4~6℃) treating callus 30mins or longer , flavonoid content hardly varies. It means the stimulation of subambient temperature to flavonoid production lasts short time. The first ten days of cell of growth is in the lag phase, and the later five daysis in exponential phase, and from the sixteenth day cell growth enter into decline phase. So the tenth day and fifteenth day are chose to determine the growth rate and the flavonoid content. The different concentrations of Cu2 + are added to solid media and liquid media to culture the callus of Ginkgo biloba L.; and with the concentrations of Cu2+ increasing, the cell growth can be slightly inhibited by Cu2+ and the flavonoid content of the examples enhances; and the action is more significant after adding precursors(0.05 g/L phenylalanine and 0.2 g/L sodium acetate). The result shows flavonoid production will be increased from one time to two times, compared with the control. Precursors can also counteract the inhibition action of Cu2+ to cell growth. Considered the two actions of Cu2+, the concentration of 10-4g/L Cu2+ is proper. And Cu2 + can be regarded as a proper abiotic elicitor. Salicylic acid can improve flavonoid production of cells in liquid medium, and the mechanics needs further research..