Effects Of Fluoride And Iodine Deficiency On The Growth And Cerebral Functions In The Filial Generation Of Rats

[Objective] The mechanism of learning and memory impairment in rat induced by fluoride and iodine deficiency and correlation of fluoride and iodine were studied, to provide the important and scientific basis for the specific prevention of fluorosis and iodine deficiency.[Method] Filial generation of rats were used as experimental objections, which parents were Wistar rats treated with fluoride, iodine deficiency, and fluoride and iodine deficiency respectively. Groups fluoride drunk double-distilled water added NaF l00mg/L. Groups iodine deficiency fed forager from the area of low iodine. Groups fluoride and iodine deficiency drunk double distilled water added NaF l00mg/L and fed forager from the area of low iodine. When filial rats were at 0,10,20,60,90-day-age, the step-down test was performed to measure the learning and memory abilities of rats, and the changes of pathological state in cerebrum were observed by microscope. At the same time, some related biochemical items were detected.[Result] Rusults of analysis showed that fluoride and iodine deficiency had effects on learning and memory abilities, pathological state in cerebrum, and all neuro-biochemical items of rats at different ages. The effects were more significant at the stage of development.(1) There are a few of nerve cells , which are Chromatin dense stain, pyknosin, and cell figure eligible , in the cortex and hippocampus of brains of rats in groups fluoride and Groups iodine deficiency, there are Chromatin dense stain, pyknosin, and peak tree projecting elongate in the large of nerve cells of rats treated with fluoride and iodine deficiency , particular during the stage of development.(2) Contracted with normal rats, EN1 and EN2 of the experimental filial rats added significantly (P<0.05). And ST reduced obviously (P<0.05). EN1 and EN2 of the experimental filial rats in groups fluoride and iodine deficiency were the highest in all groups (P<0.05). And ST reduced obviously (P>0.05 ).(3) In the groups treated with fluoride, body weight, the activity of TchE and MAO were decreased. Body weight and the activity of TchE were obvious lower than the controls at 10-day-age (P<0.05). Brain weight, MDA, H₂O₂, NO and the capacity of total antioxidation were decreased firstly, and then increased. Brain weights were decreased significantly at 10,20-day-age (P<0.05). At 10,20-day-age, the change of brain weights was opposition. NO were decreased obviously at 10,90-day-age (P<0.05). Thyroid gland weight, SOD, CAT and NOS were increased firstly, and then increased. And Thyroid gland weights were decreasedsignificantly at 10-day-age (F<0.05); NOS were increased obviously at' 0-day-age (P<0.01). A day-age, the change of NOS was opposition.(4) In the groups done with low iodine, body weight , brain weight, the content of protein , the activity of TchE and NO were decreased. Body weight at 10-day-age and brain weight at 0-day-age were decreased significantly (P<0.05); the content of protein at 10,20-day-age, the activity of TchE at 0,90-day-age and the activity of NO at 10,20-day-age were decreased obviously (P<0.05). Thyroid gland weight, the content of MDA, CAT and the activity of NOS were increased firstly, then decreased. And the content of MDA at 0,10,30-day-age and the activity of NOS at 0,10,60-day-age were increased significantly (P<0.05); The activity of CAT at 10,20-day-age were decreased obviously (P<0.05). The capability of total antioxidation, the activity of SOD and the content of H₂O₂ showed the tendency: being increased-decreased-increased.(5) In the groups treated with high fluoride and low iodine, body weight, brain weight, Thyroid gland weight, the content of protein and H₂O₂, the activity of NOS showed being increased firstly, then being decreased. And the body weight at 0-day-age and the content of protein at 0,10,20-day-age were decreased significantly (P<0.05); Brain weight at 20-day-age and the activity of NOS at 30,90-day-age were increased obviously (P<0.05); The content of H₂O₂ were d...