| The effects of Fructo-oligosaccharides (FOS) and Mannan-oligosaccharides (MOS) on immune response, lipid metabolism and performance were studied in weaned pigs. Fourty-eight 35-d-old weaned DurocxLandrace-Yorkshire pigs were divided into four groups with three replicates, each of which including 4 piglets, in a randomized block design. The four groups were fed with basic diet containing 0.5% FOS(group FOS), containing 0.3%MOS(group MOS), containing 0.45%FOS and 0.25MOS(group F+M), containing 75mg/kg Chlorcycline and 40mg/kg Roxarsone(group ABT), respectively. The results showed that: (l)Though average daily gain(ADG) and average daily feed intake(ADFI) in group FOS, MOS, F+M were lower than that in group ABT(P > 0.05), feed/gain(F/G ) in group FOS, MOS and F+M were improved(P > 0.05); The diarrhea frequencies in group FOS(0.88±0.22), MOS(1.04±0.15), F+M(0.95±0.25) were marked lower than that of group ABT(1.77±0.21)(p < 0.01). (2)The activities of total superoxide dimutase(TSOD) in group F+M(222.67±88.31) was significant higher than in group ABT(47.33±42.57) (p < 0.05) in 28th day after weaned; Compared with antibiotics, FOS, MOS, F+M enhanced the activities of TSOD in 7th, 14th, 21st day after weaned(P >0.05); There were no significant difference of the activities of catalase(CAT) among group FOS, MOS, F+M(P > 0.05); The activities of glutathione peroxidase(GSH-Px) in group FOS, MOS, were increased by 12.94%, 16.95%(p < 0.05), in contrast to that in group ABT in 28th day after weaned, at the same time, the activities of group GSH-Px in group FOS, MOS, F+M were higher than that in group ABT(P > 0.05) in other day after weaned; The contents of malondialdehy(MDA) in group FOS(7.61±1.93), F+M(3.69±2.18) were lower than that of group ABT(9.78±2.14) (p=0.01) in 14th day after weaned ; Compared with antibiotics, FOS, MOS, F+M significantly reduced the contents of MDA by 33.86%, 31.48%, 32.28%(p < 0.01), respectively, in 21st day after weaned ; FOS, MOS and F+M had no profound effects on the contents of MDA(P > 0.05) in 7th, 28th day after weaned. (3)The index of thymus, spleen, lymph node in group FOS, MOS, F+M were higher than that in group ABT(P > 0.05). (4)In 7th day after weaned, the concentrations ofimmunoglobulin(IgA) in group FOS(0.32±0.08), MOS(0.32±0.04), F+M(0.32±0.07) significantly higher than in group ABT (0.20±0.01) (p < 0.05), at the same time, the concentrations of IgG in group FOS(3.50?.49) were higher than that in group ABT(2.68±0.47) (p < 0.05); Compared with antibiotics, F+M increased the concentration of IgG markedly(p < 0.05) in 21th day after weaned; MOS increased the concentration of IgM profoundly(p < 0.05) in 21th day after weaned. (5)In 7th day after weaned; the percentages of CD4+ of thymus, spleen, lymph node in group FOS(37.33±4.04, 30.33±8.73, 37.67±2.52) were higher than in group ABT(21.33±6.03, 19.67±2.08, 24.67±4.16 accordingly) (p < 0.05), the percentages of CD8+ of spleen in group F+M{22.66±3.05) were higher than in group ABT(17.00±1.00) (p < 0.05), the percentages of CD8+ of thymus in group MOS(29.67±5.51)were higher than that in group ABT(20.67±3.51), the percentages of CD8+ of lymph node in group F+M(20.66±6.42) were lower than that in group ABT(34.33±5.03) (p< 0.05); In 28th day after weaned, the percentages of CD4+ of thymus in group FOS(32.00±5.29), MOS(37.33±3.21), F+M(32.00±3.60) were higher than in group ABT(21.33±4.04) (p < 0.05), there were no significant differences among group FOS, MOS, F+M and antibiotics(P > 0.05) in CD8+ of thymus, spleen and lymph node(P > 0.05); Though there were no significant difference among group FOS, MOS, F+M and ABT, CD4+/CD8+ ratio in group FOS, MOS,F+M was larger than that in group ABT, except that lympho node CD4+/CD8+ ratio in group FOS(1.42±0.32), MOS(1.52±0.46), F+M(1.51±0.30) was larger (p< 0.05)than that in group ABT(0.71±0.03) in 7th day after weaned and spleen CD4+/CD8+ ratio in group FOS(1.36±0.21), MOS(1.34±0.16) was larger(p=0.01) than in gro... |
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