Study Of Taxol Metabolic Regulation Mechanism In The Cell Culture Of Taxus

Taxol is a new type of anti-cancer medicine. But the concentration of taxol in taxus plants is relatively low. Furthermore, there are many by-productions in distilled substance of plant or cultivated cells. HPLC has advantages on testing these compounds. It indicated that the relationship between the peak area and the concentration was linearity. The criterion cure of taxol was C = 2. 4 10-8 S-0. 002 Baccatinâ…¢ C=2.9 10-8 S-0. 014 .Growth hormones have an important effect on growth and metabolize of cell. High concentration of NAA was disadvantageous to the form of taxol. It had few effects on the growth rate when its concentration was reduced. The content of taxol was increase remarkably when NAA:6~BA is 2:0.5 at the same time there was only a little decline on growth rate. Higher biomass accumulation and taxol yield were observed in higher inoculate volume though the growth rate was lower.The cell activity was stable in growth cycle and independent to Bacctinlll exosmosis. It is the appropriate time to subculture when the cell activity begin to drop. pH has effects on cell growth and metablism. During the growth of cells, the pH of medium decreased in the begining and increased then.We can reduce the Bacctin â…¢ exosmosis and increase taxol accumulation by such methods as follows: add MES to medium, reduce osmotic pressure and add precursor of said chain.The ultrastructure and accumulation of taxoids were correlative.6 -The ultrastructures in cells of high level of metablism were same: cytoplasm was strong and abundant, plastids were numerous. The relationship of accumulation of productions of secondary metablism and the differentiation of plant tissue will be clear if appropriate materials were used.