Identification And Genetic Analysis On Characterization Of The Protruded Style Mutant In Kenaf

Four protruded style mutant (PSM) cross-self lines have been developed in the offspring populations of space flight on Kenaf . The flower characters morphology, growth stage, the bagged seed-setting rate and rate hybrid of four PSM lines were identified, compared with common Kenaf; The response to day length and temperature of PSM was tested through the experiment with artificial short days treatment and sowing in different place; From the crossing four crosses including six basic generations were generated for studying the genetic behaviors of the mutant character -protruded style. The results showed that:(1) The flowers of PSM were small obviously. The style and the stamen tube of PSM were all short than the common, but the short range of stamen tube was higher then that of the style, and the style -obviously was longer than the stamen tube in PSM, so that the stigmas were protruded significantly from the stamen tubes. The extruded length of style was obviously different among the PSM. H20-1, which was 11.6mm, was the longest. H212-1, which was 6.5mm, was the shortest. H2-1 was 10.6mm, and H29-2 was 9.6mm. Their plant morphology were also very different.(2) The complete growth stage of PSM were 140-150 days, and were the type of early maturing. In four PSM cross-self lines, the longer the extruded length of style was, the lower its bagged seed-setting rate was and the higher the rate of hybrid seed was; On the contrary, the former was higher and the later was lower. That is to say, the protruded style contributed to receiving exotic pollen and cross. Compared with CK, the emergence percentage in the yield of four PSM all were lower, budding and flowering date were later, plant high and diameter were worse.(3) Four PSM flowered early obviously when they were treated by short days, and were sensitive to day length and temperature. Day length and temperature affected the protruded style length of PSM significantly. High temperature and long days lengthened the protruded style obviously. When sowing in different place, the mutant character inHainan was shorter than that in Jingzhou. In different years, the character were variation obviously with the change of temperature.(4) The length of protruded style have significant difference between cross and reverse cross of hybrid F1, F2, BC1 and BC2; BC parents affected the character of the offspring significantly; Segregation of the character in four F2 populations tended to its mother, but there were different obviously. It was concluded by interaction of nuclear gene and cytoplasm. Its genetic behavior had significant female effect and was complicated quantitative trait.(5) The h2B and h2N of the protruded style character were low generally, and the former was bigger outstanding than the later in many crosses, and the h2N was negative value largely. The estimate of six parameters showed that the addition all was positive value in crosses, all negative in reverse crosses; The dominance additionxaddition and additionxdominance all were negative, and the dominancexdominance all were positive. Synthetic analysis indicated that the protruded style character of PSM on Kenaf was affected easily by environment, the addition was low and the non-addition was high.