Genetic Relationship Of Peach, Plum, Apricot, Mume And Cherry Based On Microsatellite Markers

Microsatellite or Simple Sequence Repeat is a newly appeared molecular marker. Because of its stabilization, high polymorphism, and co-dominate, it was used widely by investigators. Because no research was done about mume microsatellite, and because genetic relationship of peach, plum, apricot, mume and cherry was not clear, following investigations were done in this paper.1. Because of the strong affinity of biotin for streptavidin, we use Streptavidin MagneSphere Paramagenetic Particles combined with biotin-labbled simple sequence anneal with simple DNA digested by HindIII and BaniH I H to capture microsatellite in chromosome DNA from 'jiazhouxiaomei". In this way, microsatellite-enriched library was established and Microsatellite contained DNA was sequenced. we obtained 17 microsatellite sequences with GA/CT repeat. Nine primers were designed by soft Primer 5.0. Polymorphism of these primers were shown on 24 resources, include peach, plum, apricot, mume, sweet cherry and apple. Eight primers in nine showed polymorphism alleyes.2. Thirty pairs of SSR primers from peach, cherry, and mume also, and 22 ISSR primers were used to inverstaged relationship of 'stone-fruit' trees. 27 of 30 SSR primers and 8 of 22 ISSR Primers showed polymorphism on these germplasms.3. Use soft SPSS11.5, a clustered dendrogram of 'stonefruits' was obtained. The clustered dendrogram showed that mume and apricot was the nearest among the five, they was clustered into a group first when variation coefficients was 0.522, Plum, apricot and mume were clustered into one group when variation coefficients was 0.542. Cherry, peach and plum, apricot, mume was clustered into three independent group when variation coefficient was between 0.542 and 0.555. The five trees was clustered into one group when variation coefficient reached 0.555. We find that the genetic variation of peach was narrow, while cherry was wide.