Study On The Application Of RAPD Technique To The Seed Purity Test Of F₁ Hybrid Of Balsam Pear And Pepper

The achievements in the studies of vegetable seed purity test at broad and home were analysed. The results showed that RAPD technique in suited to the test of seed purity because of its convenient, sensitive reaction more markers ,limitless primer and a few templates.A handy way extract DNA was studied by two balsam pear varities (No.3 Bilv and No.2 Bilv), i.e improved CTAB method .An optimum reaction system for RAPD analysis was set up. In the 25μL reaction system ,the concentration of DNA,Mg2+,Taq DNA polymerase and dNTPs is 20ng, 1.5mmol/L,1.0U,0.2mmol/L respectively.Based it on, the Genomic DNA of two parents of "No.3 Bilv" were tested by RAPD analysis. The results showed that primer OPQ-01 could form varied differences in RAPD maps of No.3 Bilv and their parents. It could also be appliedto the test of seed purity of No.3 Bilv. The results of RAPD test on a seed sample were completely consistant with that of seed purity testing in the fields.Primer OPS-01 which can distinguish F1 hybrid of N0.4 Yuejiao and their parents were screened from 60 random primers by RAPD method . Its ability to test F1 variety purity was proved by single plant test.