Isolation And Identification Of Pathogen Of Hemorrhagic Enteritis From Cervus In Shanxi Province

Hemorrhagic enteritis from Cervus, which is a acute infectious disease, is chiefly caused by Clostridium perfringens. The disease significant characteristics are that onset of illness is urgent, course is short, and fatality rate is very high. 8 Clostridium perfringens isolates were obtained by culturing and isolating bacteria from the viscus and intestinal contents of 11 cases caught hemorrhagic enteritis in Shanxi Province. The pathogen of hemorrhagic enteritis, which is caught by Cervus, is mainly Clostridium perfringens A, approximately 87.5%, and the sprinkle is Clostridium perfringens C, approximately 12.5%. Small intestine mucous membrane was completely necrosised and abscised, and the tela submucosa was seriously haemorrhaged by observing pathological changes of the viscus of the dead Cervus in histology. The toxin produced by Clostridium perfringens had not been decomposed and ejected from the Cervus body because the liver and kidney were damaged, which caused toxemia to die rapidly, so the symptom was hemorrhagic enteritis. Polymerase chain reaction (PCR), a rapid and effective experiment technique of molecular biology, has begun to apply in diagnosis disease and identifying bacteria and virus. Among many toxins produced by Clostridium perfringens, -toxin, -toxin, e-toxin and -toxin are the most important. According to four toxins gene sequences reported, four pairs of primers which were designed and synthesized were based on the published sequences of the four-toxin genes by computer analysis, and the rapid diadynamic method was established in the study. The sensitivity of multiplex PCR could detected 1.0 104CFU/g. There was no amplification to be observed in other types of bacteria. The preliminary application indicated that multiplex PCR was suitable for the diagnosis and survey of Clostridium perfringens.