Studies On The Protein Changes During Petal Senescence Of Osmanthus Fragrans

Osmanthus ( Osmanthus fragrans Lour.) is one of the traditional ornamental flower and is wildely used in gardens because of its specific fragrance .With the breed of large-flower varieties, osmanthus has become a kind of cut flower resource with good developing prospect. But osmanthus ornamental period is shortened by its short florescence, and this limits its development as cut flower. Nowadays, controlling the senescence of petal to prolong florescence by genetic engineering has obtained great development. This experiment established the SDS-PAGE experimental system for proteins of petal from osmanthus(O. fragrans 'Houban Jingui'), and studied the senescence of petal of osmanthus in protein level, which will bring some information for osmanthus genetic breed.The main results in this experiment were shown as follows:1. The determination of protein extraction method for electrophoresis. Based on studying and comparing other methods, an improved acetone-sediment method was achieved. By this method, the results of SDS-PAGE had clear protein bands with little background.2. Establishment of SDS-PAGE experimental system for petal proteins of osmanthus. After comparing the results of electrophoresis, the conditions of electrophoresis were modified : concentration of running gel was 11% with length of 9-10cm .the concentration of stacking gel was 3.9% with 1.5-2.0cm, the amount of added protein samples were 10ul in the vents of 10 X 2.5 X 1mm3, the running time was 2.5 hours or so. and gels were stained with the protocol selected from several staining methods. The proteins bands were clear and its background was low in the results of electrophoresis. Based on this, some preliminary study on 2-Dimensional electrophoresis for proteins of petal was carried out.3. The results of SDS-PAGE of proteins during senescent petal of cut osmanthus. The experiment analysis showed: 39. 8 kD and 27.5 kD peptides were stable from beginning to the end, 37.6 kD peptide decreased gradually and 63. 1kD, 57. 5 kD, 51.5 kD peptides appeared and increased with the senescence of petal. 51.5 kD, 32.3 kD paptides changed after ethylene and ABA treatment. This implies that there is some relationship between changes of peptides and senescence of petal.