Studies On Ultrastructrue And Comparison Of RDNA Its Region Of Some Species Of Liriomyza (Diptera: Agromyzidae)

The vegetable leafminer, Liriomyza that distributes all over the world is a category of most important pests of vegetable and flowers in many countries. In Liriomyza, L. sativae, L. huidobrensis are ultimately objects subject to quanrantine in many countries and areas. Leafminers distribute widely in our country, and make great damage in vegetable and flowers production. To solve problem that existed in methods of classifying and identifying leafminers in our country, and provide scientific basis for preventing and curing the leafminers. Through electron microscope, biochemical and molecular biological technology, we studied the classification of L. sativae, L. huidobrensis and L. brynosis, and we also researched the differentiation of host and geographic populations of L sativae in China.Through observing microtexture of L. sativae, L. huidobrensis and L. brynosis, we found there were obvious differentiation in furca and its form of arrangement in wings costa and aedeagus of male adult of three leafminers; through observing poststigma of larvae and figure of eggs, we found that the function of classification identification was not as same as in optical microscope, but the figures of eggs were obviously different in electron microscope. We also found there was difference in ovipositor's surface patterns and the figures of geographic populations of L. Sativae. So we think that the ultrastucture characters of wing costa and eggs stings could be seemed as important basis for identifying different species of Liriomyza; the ultrastucture characters of ovipositor could be seemed as basis for identifying population divergence of Liriomyza sativae.There were obviously differentiations in outcome of PAGE of L sativae, L huidobensis and L. bynosis. In Esterase Isozymes spectrum, L. sativae had 5 strips, L. huidobensis had 3 strips and L. bynosis had 6 strips.We determined and compared the rDNA ITS sequence of I. sativae, L. huidobrensis and Chhramatomyia harticola, we found that it was closed in relationship of affimity of L. sativae and L. huidobrensis, the homogenous was 98.3%; and the homogenous of L. sativae and Chhramatomyia harticola was 89.3%, the homogenous of L. huidobrensis and Chhramatomyia harticola is 89.6%.Comparing the rDNA ITS sequence of L. sativae collected from Jiangsu privince (Yanghzou), Zhejiang province, Guangxi province and Hainan province, the outcome indicated that they were closed orderly in relationship of affimity: homogenous of Jiangsu and Zhejiang was 99.1%, homogenous of Jiangsu and Guangxi was 93.3% and homogenous of Jiangsu and Hainan was 84.8%. All of these illustrated that hereditary divergence degree of geographic populations changed with the divergence degree of the geographic conditions.The rDNA ITS sequence of L. sativae collected from different hosts also were compared. It could be found that it is closed in relationship of affimity of hobby hosts-cowpea; tomato and edible gourd (homogenous of tomato and cowpea was 99.7%, homogenous of tomato and edible gourd was 95.3%) and same in non-hobby hosts-cotton and Chinese cabbage (homogenous was 94.5%) .