| Cucumber is one kind of favorite vegetables and plays an important role in vegetable supply, but it was suffered from many kinds of Fungi easily, which can decrease its yield and deteriorate its quality. Traditional method of breeding has many disadvantages, such as long period, huge investment and low efficiency. It is also restricted to the shortage of resistant genes. There is devolution of resistance in resistant cultivar. Cucumber exist serious hamper of sexual hybridization. So it is difficult to obtain the superior cultivar with high resistance. This study established a highly efficient regeneration system of cucumber and then transfer the fungi-resistant gene (bean chitinase gene) into cucumber, which mediated by Agrobacterium tumefaciens. This has not been studied in previous reports in China. The integration of target gene into cucumber genome was analyzed. The factors influencing regeneration rate and transformation rate were discussed.The results was summarized as follows:1 Effect of explants and concentration of 6-BA on organgenesis The best explants was cotyledonary nodes; The optimum shoot inducing medium is MS+6-BA0.5mg/L.2 The optimum procedure in cucumber transformation as follows: The cotyledonary nodes was pre-cultured for 1~2 days, co-cultivated in medium including 6-BA 0.5mg/L and AS l00$mol/L, pH 5.2. The optimum concentration of kanamycin used in resistant shoots selection was 30mg/L.3 Detection of transforming regenerated cucumber 30 of 78 regenerated plants of "Chang Chun Mi Ci" were positive and the percentage is 38.7%. The results of PCR demonstrated the integration of target gene into cucumber genome primarily.
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