| Fifty-three strains of Lentinula edodes collected from 13 provinces distributed in 8 floristic regions of china were assessed for genetic diversity using the RAPD technique. A total of 147 RAPD bands were amplified using 10 random primers. Variations in RAPD banding patterns and DNA similarity among the strains revealed that there is significant genetic diversity in natural population of L.edodes in china, among which the diversity of those in the Hengduanshan, the Yunnan plateau, Taiwan and south china is more abundant. A clustering dendrogram was constructed using average linkage clustering. The 53 strains were classified into 4 groups using 0.658 similarity as a cut-off point. Group I and Group II mainly consisted of strains in the Hengduanshan, the Yunnan plateau and central china. GroupIII comprised those in the other regions. And groupIV was small branch composed of some strains in the north china and Sichuan Province. The 4 groups could subsequently be subdivided into 20 RAPD groups (RGs) at 0.750 similarity. Among them, sixteen of these RGs correlated strongly with the locality of strains, and the remaining 4 RGs belonged in widespread distributive types. |
