The Identification Of The 024-Strain Of Actimycetes And The Purification And Structural Analysis On Its Antibiotic

024 strain, which was screened from many Actinomycetes, inhibits Rhizotonia cerealis intensely which can cause Wheat sharp eyespot. Its initial and optimal fermentation condition of production of antifungal substance have been studied, and found the the optimal condition (the initial pH of medium 7.0, 100ml liquid medium in 250ml flask, fermentation temperature 28℃, 170rpm for 4 days, soybean cake culture medium) and other influencing factor such as the temperature, metal hydronium. This paper studied the identification of the 024-strain of Actinomycetes and the purification and structural analysis on its antibiotic. The 024-strain of Actinomycetes was idientified as a new species of the Streptomyces through the classical and molecular methods. The antifungal spectrum of 024-antibiotic includes many plantpathogenic fungi, such as Colletotrichum gloeosporioides, Fusarium gramiaearum, Helminthosporium sativum, Helminthosporium maydis, Verticillium dahliae, Colletotrichum gloeosporioides..Different extracting and purifing methods were taken, such as organic solvent extraction, alumina column chromatography and silica gel column chromatography etc.. The effective approach was found that, first, the 024-antibiotic was dealt with the primary methods—fermentation, filtration, condensation, disinfection and so on, then it can be purified by methyl alcohol soaking, alumina and silica gel column chromatography. The acetone, methyl alcohol, germfree water and their mixed liquor were used in different column chromatography. In the continuous column chromatography (alumina and silica gel column chromatography), there are two part which have antifungal effect on Rhizotonia cerealis . After the evaporation, we get the crystal of this antibiotic, which has strong antifungal effect.Through the advanced equipment ,such as ultraviolet radiation, infrared, the crystal of this antibiotic was identified.The result shows that the primary component is a sort of disaccharide and its molecular weight is 382.6, otherwise there is the other substance, whose molecular weight is 1153.5 and its structure is been studying.