Study About Release Of Free Amino Acids And Peptides In The Rumen Of Sheep With Diets Of Different Protein Resources

This study used in a 3×3 latin square design, and three diets, of which main protein resources are soybean, fish meal and corn gluten meal respectively to investigate the profile of amino acids ,concentration and form of peptide in the rumen of sheep The experiment consisted of two parts, the first, we anylysed the profile of amino acid of protein sources feeds for experiment, and searched degradation regularity of them in the sheep rumen, I the second we evaluated the profile of amino acid ,concentration and form of peptide of different protein sources in the rumen of sheep. Results showed that:Part 1.The free amino acid had the higher concentration in fish meal group in contrast to soybean meal group and corn gluten meal group in 6 hour after meal. His, pro and Arg had the change with higher amino acid concentration in each experimental group in 6h after meal(P<0.05); and Gly had the change with lower amino acid concentration (P<0.05).showed that His, pro and Arg were not easy degraded in rumen and Gly were easy used by bacterios.Part 2. The release of peptide and free amino acid is not related by degradation of protein source feed There are the most release of peptide in the first hour,and different proportion of peptide to free amino acid, feeded different protein sources feed. The average proportion of amino acid of peptide to total amino acid ,72.28%(soybean meal),90.99%(fish meal group),91.90%(corn gluten meal).Part 3 The AA form of peptide in the rumen are similar in each experimental group, Asp,Glu,Tyr and His had higer amino acid concentration in 6 hour after meal, in the amino acid concentration of peptides from 1h to 6h after meal, Glu,Tyr,Val and Leu Had the changes with higher amino acid concentration. .showed that Asp,Glu,Tyr and His were not easy degraded in rumen...