Construction Of Antisence Polygalacturonase Gene Plant Expression Vector And Transformation Of Cucumis Melo L.cv Hetao

The region of MPG1,128bp sequence was obtained by self-amplification of a pair of designed long primers(75bp)and was inserted into pMD18-T vector. After selecting the antisense clone in which the target gene was inverse inserted and further cloning such sequence into the downstream of 35s promotor of CaMV and "Ω" enhancer of TMV of a plant expression vector ,pBINYXW, we have constructed a expression vector containing antiseanse PG gene of Cucumis melo successfully , named pUC38-PG. Using pollen tube pathway (stylar daubing,ovary injection and stylar incision) transformed pUC38-PG. plasmid into Cucumis meloL.cv Hetao,204 fruit had been btained.The seeding rate of three ways were 39.92%,34.48% and 28.70%.There were prominent difference between stylar daubing and stylar incision...