Effects Of Cysteamine And Two Essential Amino Acids On Celluar Immunity In Goats

To gain insights into effects of cysteamine , methionine and lysine on celluar immunity in goats fed with different diets ,the following experiments were carried out.1.Effect of dietary nutrition and cysteamine pre- and post- surgeries on transformation of peripheral blood lymphocyte concentrations of serum IL-2 and cortisol in goats8 adult castrated goats fed with peanut stems, were randomly divided into 2 groups :control and experiment groups. Water was avalible at all time.They experienced 4 periods:1) Period of no supplemental dietary nutrition before surgery;2) Period of supplemental dietary nutrition before surgery;3)Period of surgery on rumen ;4) Period of surgery on duodenum. Supplemental nutrition ( 50 g /goat-d) was added to the diet of all goats; In addition ,cysteamine (45mg/kg BW-d) added only to the diet of experiment group in the last three periods.Two peripheral blood samples were collected at different times(the 3rd day of period 1, 11th day of period 2,8th day of period 3 ,4th and 13th days of period 4) respectively). One was used for test of transformation of peripheral blood lymphocyte(TLC) in 30 min,and the other was separated for serum and stored at -20 C until RIA assay of concentrations of IL-2 and cortisol. Results showed supplemental nutrition exerted significant effect on TLC( P<0.05 ) in normal physiological condition,but no effect on level of serum IL-2 and cortisol. Surgery induced the decreases of TLC and serum IL-2 ,increase serum cortisol of the control goats,but the extent of decreased TLC of experiment goats was lower than that of control;There were no changes of TLC and serum IL-2 after surgery in experiment group. We concluded the following results :1) the supplemental nutrition can improve the function of lymphocyte in goats; 2) surgery can induce the immunosuppress;3)cysteamine can dimish the celluar immunosuppress by preventing the decrease of TLC , concentration of serum IL-2 and increase of serum cortisol induced by surgery.2.Effect of cysteamine administrated by ruminal fistula or/and two kinds of essential amino acids by duodenal fistula on transformation of peripheral blood lymphocyte concentrations of serum IL-2 and cortisol in goats5 castrated adult goats fixed permanent ruminal and duodenal fistulae a month ago experienced four periods : 1) Control period:5mL saline was administrated twice per day through duodenal fistula; 2) methionine plus lysine period: methionine(0.03%dry matter ) and lysine(0.05%dry matter) , which were dissolved in 10mL saline ervery time, were administrated twice per day through duodenal fistula ; 3)cysteamine periodxysteamine (50mg/kg BW-d ) was administrated twice per day by ruminal fistula and lOmL saline by duodenal fistula at the same time ; 4)cysteamine plus methionine plus lysine period : cysteamine was administrated by ruminal fistula and two kinds of essential amino acids by duodenal fistula at the same time with two times per day;Each period lasted 14 days.They ate peanut stems and drank water ad litum. Two peripheral blood samples were collected on the llth day of each period. One was used for test of transformation of peripheral blood lymphocyte(TLC),and the other was separated for serum and stored at -20 C until RIA assay for IL-2 and cortisol. The results showed TCL of cysteamine or/and amino acid period was significantly higher ( P<0.05 ) than that of control period ,and cortisol concentration of the last periods was lower significantly (P<0.05 ) than that of control period . IL-2 concentration of experiment period was only higher(P=0.09) than that of control period under the condition of combination of cysteamine and two essential amino acids. These results indicate the effect of the combination of cysteamine and two essential amino acids on celluar immunity is more powerful than that of either of them .3. Effect of the combination of cysteamine orally and two amino acids administrated by duodenal fistula on transformation of peripheral blood lymphocyte concentrations of serum IL-2 and cortisol in goats10 castrate...