| An effective regeneration of two highblueberry variety of 'Berkly'and 'Bluecrop' and agrobacterium-mediated transformation system were developed in this study. A salt-tolerant gene named AtNHXl gene was introduced into 'Bluecrop',and new transgenetic blueberry germ plasm were obtained. The result went as followed.1. In order to found an effective propagation system for 'Berkly'and 'Bluecrop',effects of basic medium was compared. Results shows that shoots propagated well on medium improved 1/2MS added with 2iP5mg/L,the percent of multification of Bluecrop is 86.7%.the percent of multification of berkly is 90%,When the medium added IBA O.lmg/L, The percent of rooting of 'Bluecrop'is 66.7%,but the 'Berkly'is 33.7%.The percent of rooting of 'Bluecrop'is 45.5%with IBA 0.1mg/L and NAA0.1mg/L.2. Factors that effect percent of regeneration of 'Berkly'and 'Bluecrop' was studied, include types of basic medium, types and concentation of hormone, time of darkness treatment, node position of regeneration leaf, ways of leaf deposition. Processf was culture leaf on improved 1/2MS medium containing ZR 5mg/L, 1-2nodes needs no darkness culture.The regeneration of 'Berkly'is 100%and 'Bluecrop'is 96%.The regeneration system of 'Bluecrop' which have the highest regeneration rate at 96% was chosen to use in transformation system.3. Factors of transformation include time of dipping and cocultivar, types and content of antibiotic, intension of selection were studied, and effective transformation system of 'Bluecrop' was developed as following: leaves dipped into agrobacterium suspension for five minutes, then cocultured on 1/2MS with ZR5mg/L and AS 80mg/L for three days, followed with maintainable culture on 1/2MS with ZR5mg/L, Km10mg/Land Cef 250mg/L.Then resistance shoots were obtained through regeneration system.4. In this study, we obtained 'Bluecrop' resistant shoots identified by GUS dyed,PCR and PCR-Southern. Approving the AtNHXl gene integrated into host genomic DNA of 'Bluecrop'.
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