Decomposing Microbes Constitution Of Agricultural Organic Castoff And Studies On Biological Slow-released Nitrogenous Fertilizer

The author severed and filtered two thermalphilic bacteria in possession of eellulase, two yeasts depressing NH3 release capacity which can tolerate high temperature , an actinomyceto possessing laccase activity.NMF microbiological fertilizer is constituted by five strains above. Its classification status , fermentation conditions , plant security , effects on decayed animal manure and action on biological slow-release nitrogenous fertilizer are studied in this article.Bacteria were named B022, B023 respectively and yeasts Y124, Y127, actinomyceto F04.Through experiment about their configuration and physiological , biochemical characters,B022, B023 are identified as bacillus spp. And Y124, Y127, F04 are identified as Schijosacchar , Hansenula, Thermoactinomyces respectively. NMF biological fertilizer cannot incur diseases when added in paddy , wheat and tomato fields during plant security experiment.Results show that NMF fermentation liquor cannot inhibit plant pathogenic bacteria offered in this article.Through single-factor and multiplefactor experiment,the optimum fermentation medium of B022 is confirmed as starch 0.8%;glucose 0.2%;soybean powder 0.2%; carbamide 0.1%; potassium phosphate dibasic 0.01%;temperature 50 C; incept pH 7. 2; the optimum medium of B023 is starch 0.8%;glucose 0.2%;soybean powder 0.2%; ammonium sulfate 0.1%; potassium phosphate dibasic 0.02%;temperature 45 C; incept pH 7. 5; the optimum medium of Y124 is glucose 0.5%;sucrose 1.5%;yeast extract 0.2%; ammonium sulfate 0.1%; potassium phosphate dibasic 0.02%;temperature 28 C; incept pH 6. 5; the optimum medium of Y127 is glucose 1.5%;maltose 0.5%;yeast extract 0.1%; carbamide 0.1%;potassium phosphate dibasic 0.02%;temperature 37 C; incept pH 6. 4; the optimum medium of Y124 is glucose 0.5%;sucrose 1.5%;yeast extract 0.2%; ammonium sulfate 0.1%; potassium phosphate dibasic 0.02%;temperature 28 C; incept pH 6. 5; the optimum medium of F04 is starch 0.8%;glucose 0.2%;soybean powder 0.2%;sodium nitrate 0.1%;potassium phosphate dibasic 0.02%;temperature 45 C; incept pH 7.8.When cow dung was disposed, the cow dung inoculated by NMF can achieve standard after 25 days. The general nutrient and humic acid were increased by 18%, 45% respectively.NMF can hasten speed of disposing cow dung by way of alter microbiological fauna, increase laccase activity and cellucase activity.Organi fertilizer produced by NMF can satisfied GB7959-87 and relieve inhibition of germentation of rape.The content of heavy metal accord with quality criterion of commodity organic fertilizer.During disposal of cow dung,chemical nitrogenous fertilizer and NMF were added,at initial stages ,pH descended largely.The content of organic acid and humic acid were increased by 54.6%, 28.1% respectively;protein nitrogen increased by8.1%;valid nitrogen accouted for 22.3% of chief nitrogen ;valid nitrogen accouted for 48.7% of chief nitrogen when treated without inoculation.NMF would transform valid nitrogen into protein or fixed in form of organic acid ammonia and humic acid,which may be the important approach of nitrogen release.During potted plant inoculated by pathogenic microbes of wheat scab and gray mold of tomato,when treated by organic fertilizer produced by NMF,the ratio of wheat scab were reduced by 54.2 % - 59.0 % than comparision without fertilizer and compost treatment respectively ;the ratio of gray mold of tomato were reduced by 23.7% , 17.3% respectively.The reason maybe that there is nutrition for plant pathogenic microbes in compost.The microbes fauna was changed after organic fertilizer disposed.In potted pimiento experiment, after treatment by biological release nitrogenous fertilizer and compound fertilizer constituted by organic and inorganic fertilizer, pimiento grew faster and content of soil organic matter were increased compared with chemical nitrogenous fertilizer addition. Content of Vc were increased by 25.1 %, 13.0% respectively. The content of nitrate salt were decreased by 59.8%, 43.3% respectively. After treated by biological release nitrogen...