Genetic Analysis And Single Sequence Repeat (SSR) Study On Wheat Early-Maturity Mutant

Selecting from the natural variation in wheat developed early maturity, which enabled plants to escape hot and dry conditions during late spring. Early maturing wheat varieties also carry high yield potential represent a major boon for wheat producers. From many scholars' researches, it showed that maturity time is important for the adaptability of wheat varieties to different environments, and a complex network of interactions between numerous genes is responsible for the regulation of this important trait. Based on their interactions with environmental signals, these genes can be divided into three broad categories: photoperiod responsive genes (Ppd), vernalization responsive genes (Vm), and 'earliness perse' {Eps) genes. With the molecular biology techniques, more and more genes related to wheat early-maturity would be identified.In this study, the wheat variety of Jishi8810 were crossed with the early-maturity mutant Jishi8810-1, generating from Jishi8810 which treated with zhengdingmycin(0.1~1 ug/ml), the Fipopulation consisting of Jishi8810-1 X Jishi8810 and Jishi8810X Jishi8810-1 were obtained in current year, the BC1 and F2 population were established in the following year, and the F3 population was derived from the hybrids in the third year. Heading date as the early mature standard, the early mature plant and usual plant were identified. The genetic analysis revealed that the short heading date of early-maturity mutant Jishi8810-1 was probably controlled by one recessive gene.The simple sequence repeat (SSR) markers from wheat were conducted in this research. Wheat genomic DNA pools consisting Jishi8810 and Jishi8810-1 were established respectively with bulk segregate analysis (BSA). Three out of 230 pairs of test primers amplified polymorphic bands in two kinds of wheat genomic DNA pools. Two primer pairs showed the same discriminating results with replications. The microsatellite marker Xgwm499 was linked with the early-mature character, and the primer Xgwm114 was associated with the late heading date. With bio-softwareJoinmap1.4, the result indicated that the gene related to early mature character was located on 5BL and the genetic distance between the SSR marker Xgwm499 and this gene was 13.4 cM. The primer Xgwm114 that associated with the late heading date was located on 3BL or 3DS and the genetic distance was 7.1cM.