Molecular Detection And Identification Of Tilletia Controversa K(?)hn

Dwarf bunt of wheat is the most serious and difficult controlled disease which often make; great damages in the production of wheat, it is also a kind of quarantined diseasem in China. But the teliospore morphology of T. caries (TCT), T. foetida (TFL) and T. controversa(TCK.) is found very similar. So it bring us great difficult on the quarantined identification. Many research have been done on the morphology, serology and biochemistry of TCK, TCT and TFL for many years and acquired great progresses. But there are no reports that have been found on the molecular biology in China.T. caries(TCT) > T. foetida(TFL)and T. controversa(TCK) have been differentiated by using two methods of molecular biology which combine Real-time Fluorescent PCR in this paper. Primer OperonA01 have been found to differentiated TCK and TFL but can ' t to differentiated TCK and TCT by using RAPD. Special sequences have been found at the externally transcribed spacer(ETS) and at the non-transcribed spacer (NTS) of ribosome gene by analytical method of ribosome gene sequence . Taqman probe have been designed according to the special sequences of TCK ETS sequence. TCK has been detected by using Real-time Fluorescent PCR.TCK can be deteced rapidly, accurately, relatively sensitive and effectively by Real-time Fluorescent PCR which based on probe Taqman .It reduced pollution due to adopting full-closed reaction tube and photoelectricity conduct system during the experiment.