Agrobacterium-based In Vitro Transformation Of Medicago Sativa L. With The GPDH Gene Of Saccharomyces Cerevisiae

Soil salinity is a major constraint to food production because it limits crop yield and restricts use of land previously uncultivated. The United Nations Environment Program estimated that approximately 20% of agriculture land and 50% of cropland in the word is salt-stressed. Soil salinity also limits the caloric and the nutritional potential of agricultural production. These constrains are most acute in areas of the world where food distribution is problematic because of insufficient infrastructure or political instability.On the horizon are crop improvement strategies that are based on the use of molecular maker techniques and biotechnology, and be used in conjunction with traditional breeding efforts. All these breeding improvements are depend strongly on the further understand of the mechanism of salt stress tolerance of plant. More stress resistance can be obtained after improved the plant tolerance abilities artificially. Salt tolerance had been achieved by the plant transformationMedicago sativa L. is an important legume with good elite, high quality and good delicious, its rhizobium symbiosis system fixing more nitrogen, thus almost needs not nitrogenous fertilizer. Beside these use, alfalfa planting plays a important role in the areas of water and soil conservation, soil improvement, honey source providing et al. And now, we call the alfalfa "king pasture" because of its expansional planting, long history of cultivation and all the elites. Scientists had successfully transformed alfalfa varieties by agrobacterium infection with some resistance genes. We have isolated GPDH gene from Saccharomyces cerevisiae strain by gene chip technique, and also understand much about its function. The final goal is to improve the salt stress tolerance of alfalfa and make the transformed seedlings pass the new characteristics to the offspring genetically and stably.