| Bacteriostatic action of 23 chinese medicinal herbs extracts with alcohol on Escherichia coli ATCC11411,Staphylococcus aureus ATCC26112,and Pseudomonas aeruginosa was studied experimently.In these medicines,Arisaema consanguineum schott tuber extracts with alcohol had obvious action in these three bacteriostasis,then its bacteriostatic action and mechanism and its effect on colibacillosis in chiken were studied.The results showed the extracts had obvious action in bacteriostasis upon Gram-positive and Gram-negtive bacteria,and bacteriostatic spectrum was wide.Primarily analysis indicated spaonins element of its Arisaema consanguineum Schott is the principal material for Bacteriostatic action.Observation under scanning electronmicroscope revealed that the structure of Escherichia coli was destroyed by the extracts,and Escherichia coli changed shapes,lastly,constricted into little particles and died.Observation under transmission electronmicroscope revealed that cytoplasm was concentrated,cell membrane was separated from cell wall,then cell membrane was broken down into a cavity,a part of cell wall was disappeared and cell membrane was broken.The results of SDS-PAGE showed that the content of the total protein in Escherichia coli decreased,especialy the proteint of large molecule,some of them disappeared.Drafted the growth curves of normal Escherichia coli and the growth curves of the Escherichia coli effected by the extracts,by contrast,the result showed bacteriostatic action mainly produced at logarithmic phase and Bacteriostatic Mechanism was possible to inhibit cell division.By the experiment, Arisaema consanguineum schott tuber extracts with alcohol had strong bacteriostatic action upon Escherichia coli 01 C84010 and Escherichia coli k88C83905.It had practice potent of substituting antibiotic to prevent chiken colibacillosis and pig colibacillosis.The experiment of acute toxicity showed the LD50 of the extracts is 535.06～372.90g/Kg. |
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