Transformation Of Rice Waxy Gene Via Pollen-tube Pathway

Poor quality of rice grain is currently the common issues nationwide. Amylose content is regarded as the most important factor in determining rice quality. It is efficient to reduce the amylose content to adjust the waxy locus expression level, which is responsible for the synthesis of amylose in the triploid endosperm of the developing grain and in the haploid pollen.Pollen tube pathway has been successfully utilized in transgenic research work in kinds of crops, rice included. Antisense down-regulation is a useful tool for adjusting gene expression level. Rice variety, 11-32 is one of widely used restoration lines in China. Though it has shortage which is its amylose content being as high as 25.88%. Using a construction consisted of antisense fragment of rice waxy gene, 2 transformant were obtained by pollen-tube pathway. In T, generation, Southern blot analysis and histochemical checking proved the presence of waxy gene fragment. And the amylose content has been reduced to 13.48% and 17.47% in this two transformant. Furthermore, 1 and 3 plants'amylose content tends to be stable and reduced in T2 generation.