| In recent years, with the development of molecular biology, make it possible to get male-sterility line through genetic engineering. With genetic engineering, we can get male-sterility line and resume line. Moreover when it is succeed in one crop, then it can be supplied to other crops. So in recent years genetic engineering is being utilized broadly and stepwisely being tended towards into commerce. Brassica oleracea. L?it is belong to colewort of cruciferae, a mutation of Brassica oleracea. L. The heterosis of cabbage is significantly notable, for the long time Brassica oleracea. L breeding get through self-incompatibility lines.but with self-incompatibility lines to breed, there are two sides disadventage: the first is inbreeding weaken for continual self-incompatibility,not only the coefficient of propagate will lower,but also the quality of self-incompatibility will be weaken.so that make the hybridize rate fall. The second is that bud stage should be pollination with manual work, do it is very annoyed, and the efficient is low,the cost is very costliness,this will increase the cost in breed. If use male -stereile line to breed will overcome those disadvantage.Orthodox to get male -stereile line is through these ways of nature diversity manpower induce distant related hybridize and autocopulation hybridize in breeds.then may be get male -stereile line through breeding selection for several generations .This article is to transfer male stereile gene-- Anti-actin gene and barnase gene which is linked with different special .anther or pollen promoter to Brassica oleracea. L through AgrobAnti-acterium tumefaciens-mediated. Anti-actin gene link with the promoter TA29 which is cloned from tobacco and the promoter NTM19 cloned from tobacco, then construct the chimaetic gene closed linkage with the gene BAR which expression can fastness herbicide PPT, Barnase gene is also construct gene expression vector through this way. Then transfer the two genes to cabbage through AgrobAnti-acterium-mediated transformation method . The main work and results are as below:1 High efficiency regeneration system of Brassica oleracea. LThe high efficiency regeneration system is the base of plant genetic transformation. The explants was hypocotyls and MS was used as basic midium. By supplying different concentration of 6-BA, NAA, we got the optimum media which induced the adventitious bud differentiation of two Brassica oleracea. L explants types. The optimum media is MS+BA 1.0mg/L+NAA0.2mg/L.we also compare two explants of cotyledons and hypocotyls in Brassica oleracea. L.Between the two explants types, the hypocotyls expressed the higher adventitious bud differentiation capacity.In the same time,we try on different concentration IAA on the 1/2 MS media,the result show that no way how much the concentration is ,the Explants can get root,but the time is longer with the concentration lower.2 The best PPT concentration used to select the transformant plantsExplants were restrained from differentiating when 2.0mg/L PPY was added to the medium. When PPT concentration was 2.5mg/L, all of the explants and callus became white and dead. The adventitious bud put in medium contain 3.0mg/L PPT will be dead mostly, in PPT 3.5mg/l medium cause the adventitious bud all dead,but in root medium.PPT1 .5mg/L can cause the adventitious bud not generat root-So the best selective concentration of PPT is 2.5mg/L,The bud selective concentration of PPT is 3.5mg/L,the bud generat root selective concentration of PPT is 1.5mg/r .3 Transformation system of cabbageThe procedures described were derived from numerous regeneration and transformation designed to test fAnti-actors that might affect shoot regeneration. Tested parameters include length of preculture length of inoculation with agrobAnti-acterium , length of co-cultivation select which antibiology and AS. Only those parameters producing the best result are described as below:The cabbage explants which get from 6-7d were precultured on regeneration medium. After...
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