| Avian infectious bronchitis is an acute, highly touched infectious disease caused by infectious bronchitis virus, which is one of the disease threaten poultry industry. Our study is to establish a convenient, specific and rapid detective technique for routine measuring antibodies to infectious bronchitis virus. Method: IBV was a propagated in the allantoic cavity of 9-day-old specific pathogen free(SPF)embryes, as well as inoulated in the monolayer of chicken embryo fibroblasts(CEF) by serial passage. Followed purification, then measuring protein quantity, which is used coated antigen. Conjugation was to label the HRP to rabbit anti-chichen IgG using periodate method,TMB was as substrate, to set up enzyme-linked immunosorbent assay(ELISA) for testing antibodies to infectious bronchitis virus. Results: IBV obtained by culturing in the CEF monolayer could be used to the coating antigen. No crossing reaction was observed with antibodies to Newcastle disease virus(NDV), Marek's disease virus(MDV), infectious bursal disease virus (IBDV), infectious laryngotracheitis virus(ILTV), Avian influenza virus(AIV), Avian parainfluenza virus(APIV), Reticuloendotheliosis virus(REV), Infectious Coryza(IC), Mycoplasma gallisepticum(MG), Mycoplasma synoviae(MS). IBV antigen having a better group specif icing could be reacted with antibodies to IBV Gray strain M strain. Conn strain, T strain, B strain, H strain, Ark99 strain, SE17 strain individually. The coincidence of result was good by comparing with the imported reagent of IB-ELISA produced by IDEXX. Conclusion: Our developed IB-ELISA detective reagent can be used to test antibodies to IBV. |
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