Studies On Mechanisms Of Microecological Preparation's Strains In Biocontrol Of Wheat Sharp Eyespot

2011 (B. cereus), 2012(5. cereus), 2013(5. cereus) and 2014 (5. subtilis) strains of the beneficial endophytic bacteria screened from wheat, could prevent and cure wheat sharp eyespot. The four strains, were marked with streptomycin and rifampin. The colonization in wheat root, stem-base and leaf was investigated through diluting method. The results showed that the target strains could colonize internal leaf tissues inoculated by the four strains and transfer to internal stem-base, the other leaves and root, and could also transfer to stem-base and leaf of wheat infused by the four strains suspension into imbibed seeds. High concentration of their suspensions, long time of seeds imbibed and positive temperature in growing were in favor of the colonization endophtic bacteria in wheat. It could be seen from the study of orthogonal arrays that the suspension was the best factor in colonization of endophytic bacteria in root.The four strains were cultured on PDA against sharp eyespot of wheat.The result showed that 2014 strains and the metabolite were efficacious against Rhizoctonia cereal is. It was an antagonistic band about 15cm between 2014 strain and R. cerealis. An antagonistic band was not observed between 2013 strain and R. cerealis, but the metabolite of 2013 strain could be against R. cerealis. 2011 strain and 2012 strain were covered by R. cereal is and the metablite of them could not be against the growth of R, cerealis.The metabolite of the four strains was separately put together with the wheat seed. The metabolite of 2011 strain, 2012 strain and 2013 strain could increase the germinate rate and growth of wheat, But the metabolite of 2014 strain could not increase the germinate rate and growth of wheat.