CDNA Cloning And Identification Of Benzoyl-CoA: Benzoytransferase From Rice Induced By Magnaporthe Grisea

Plants show a series of complex physiological and biochemical changes in response to pathogen attack. Most of these responses are mediated by activation of plant genes including various defense genes and those involved in the process of pathogen recognition, signal transduction and initiation of transcription. The functional identification and expressing characterization of plant genes that are induced during interaction with pathogen will be a great help to reveal the mechanism of plant-pathogen interaction; isolation of these genes may provide a good choice for engineering plant disease resistance.Phytoalexin is utilized by plant to resist biological or none biological invading factors, which is secondary metabolic product. It is an important fungi resistant material that have activity in vitro and can be inducible by invading factors. It is thought to be an important mechanism for its induced synthesis in plant after infected by pathogen. Fan and Peng(1996) isolated two phytoalexin in rice inoculated with incompatible race of Magnaporthe grisea. They accumulate in rice after 48 hours. According to their chemical structure, benzoc acid and cinnamic acid is precursor of this two phytoalexin. The density of JA immediately increase after elicitor., is correspondent with process of phytoalexin induction. It can be inferred that Benzoyltransferase involved in rice resistance reaction to Magnaporthe grisea.The author characterized one full-length cDNA. Sequencing result showed that the full-length cDNA which consists of 1610bp has an intact ORF (82-1419) that encodes an 446-amino-acid protein. BLAST search indicated that the deduced amino acid sequence shares 41.69% identity with that transferase of from Hordeum, Taxus chinensis,.Catharanthus, Diabnthus caryophyllus respectively. Then there is a different band with 74kDa in the SDA-PAGE , after indued with IPTG .the result of expressing Benzoyltransferase in E. coli showed Benzoyltransferase has the transferase activity. Benzoyltransferase transcript in rice leaves can be induced by blast fungus infection.