Studies On Transfer Of Glyphosate Resistant Gene Into Maize Inbred

This study mainly dealts with the transfer of glyphosate-resistance gene (EroA) into the elite maize inbred line of Zong 31 by using gene gun, the molecular identification of TO , TI generations and the field identification of T1. In addition, the field identification was based on the investigation result of glyphosate tolerance of Zong 3land weeds and the law of the weeds naturally occurrence in the field. The main results were as follows:l.The kinds and the occurrence peak of weeds were investigated by five-spot-sampling method. There were 11 kinds of weeds in the maize trial field.2.The development comparison between weeds and the maize verified that the weeds occurrence peak was in the 3-leaf and 4-leaf period of Zong 31 .The tolerance to glyphosate of Zong 31 and weeds in the 3-leaf period indicated that it was belter to prevente and cure weeds when the glyphosate concentration was 1.2g/l,which was nearly the lethal concentration of Zong 31 in the 3-leaf period.3 The effect of different culture mediums on the different callus, which were different in the times of subculture, was investigated.The result of the "F" culture medium is better than others.4.The glyphosate resistant gene, RA was transferred into Zong 31. Only 456 generated plants were gotten seeds, because of bad weather of continuous high temperature in greenhouse. Results from PCR, PCR-Southern, Southern blot and RT-PCR showed that the glyphosate resistant genes were integrated into the maize genome and could be transcripted in 153 TO plants, in which there were!34 plants were gotten seeds.5.The effect of selective time was investigated in the callus, which showed that about one month was better. Because the callus in the condition kept stronger differentiation abilities but the most of control callus died and brownized, which was advantage for the plantlet.6.The plants of Tj generation and inbred Zong 31 were spraied with glyphosate of 1.2g/J on leave surfaces in the 5-leaf period. Due to the typhoon and the other factors' effect, ten plant were remain, and only get two ears.7.The results of PCR, PCR-Southern and Southern blot of the plant potted in the room also confirmed that the glyphosate resistant gene was transmitted to Tl generation.