| The BALB/C mice were immunized with the inactivated streptococcus suis type 2 by 0.8% formaldehyde.When the serum anti-MRP-antibody titre came to 1:104, the spleen cells were fused with the SP2/0 by PEG-1500. Indirect ELISA was founded to detect the hybridoma surprunt by purified expressed MRP with the His-Bind Resin . Eight hybridoma strains secreting McAb against MRP were obtained by limited diluting several times and the ascites was identified with Westen-blot. A double antibody sandwich ELISA was established to detect streptococcus suis type 2.A Formalin-killed whole bacteria cells of HA9801 antigen-based enzyme-linked immunosorbent assay(WCA-ELISA) to detect antibodies against Streptococcus suis type 2 was developed and compared with a purified capsular polysaccharide antigen-based indirect ELISA (CPS-ELISA). Although the standardized gave satisfactory results that most cross-reactions decreased significantly, the result of positive sera of animals associated with Streptococcus suis type 2 and the result of negtive sero by CPS-ELISA did not present liters significantly different. According to the results, it could be concluded that the CPS-ELISA developed in this study used as a diagnostic tool to identify infected animals was discredited. Specificity is improved by diluting the sera with WCA-ELISA. A appropriate dilution can be found out to make the result of detection accuratest. It is proved that the result was more reliable with WCA-ELISA than with CPS-ELISA.70 shares of positive sera and 70 shares of negtive sera diluted to100, 200, 400 , 800, 1600 were tested with a whole bacteria cells of HA9801 antigen-based enzyme-linked immunosorbent assay. The result was analysed to determine the appropriate dilution and the criterion to makesure the negtive sera and the positive sera by epidemiology. It proved that: Beforel998, there was the infection ofStreptococcus suis type 2 in Jangsu province by detecting the sera collected in 1997.
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