| The X stage avian blastodermal cells have not yet differentiated, and when they were introduced into the same stage embryos of recipients. They could take part in the development of different tissues of the recipients. In the experiment, the langshan is used as the donor and the AA broiler is the recipient, the langshan-AA chimeras were produced via windowing the recipient eggs. 108 eggs were manipulated, and 19 chickens were hatched, the hatching rate was 17.6%. 22 chimeric embryos were obtained, and the chimera rate was 20.4%. 15 chimeras survived, and the survived chimera rate was 78.9%. Abnormal testises were founded in one of the two male chimeras by dissecting. Hetero-sexual cells were found in the gonads of both chimeras by PCR amplifying the W-specific repeating sequences. The result showed that it was possible to produce transgenic avians by using our method of producing chimeras.By observing the 10 behaviors of the langshans, the AA broilers and the chimeras, Significant differences (p<0.01) were found on standing, sitting, stand preening, stand environment pecking, walking or running between the two control breeds, and the frenquency on these behaviors of chimeral were all located in 95% confidence intervals for means of the langshan, so its behavioral patterm prefered for the donor langshan. Chimera2 and 4 were similar to the langshan chickens separately on the performance of dustbathing and standing, while the behavioral pattern of the other three chimeras were close to the recipient. The method of producing chimeras can also be used in avian neuroethology.Plasmid pEGFP was introduced into the X stage avian balstodermal cells via lipofectamine in vitro, the exogenous gene began to express. After cultured for 12h in vitro the transfecting rate reached 21.43%. The transfected blastodermal cells were injected into the subgerminal cavity of the recipient, after bing hatched for 6 days the exogenous genewere detected in 40% (8/20) of the embryos examined.
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