| The experiment was conducted to study effects of Conjugated lionleic acid (CLA) on growth performance ,carcass quality and lipid metabolism in different breeds and sex of broiler chickens by determing related common indexes . Molecular biology method (RT-PCR) was also used in the study to analyse mRNA expression of LPL> SCD, PPARa, PPARr enzyme genes which related to lipid metabolism ,as well as the machenism of CLA was also studied in this experiments .Results were as follows :Average daily gain for l-21d ,22-42d and l-42d increased by 0.87%,6.31%,5.0% for AA roosters and 3.86% , 10.42% , 8.69% for "Changsha" yellow roosters Respectively , compared to correspanding control groups . But average daily gain for AA hens decreased by 0.74%, 3.31% and 2.7% , respectively , compared to correspanding control group .No significant difference was examined to average daily gain among groups (p>0.05 ) .As to average feed intake of l-21d , 22-42d and l-42d, AA hens in tested groups decreased by 3.8%, 8.5% and 7.6% respectively , compared to the control group . The corresponding values for AA roosters in tested groups were 3.2%. 3.7%' and 3.7% respectively , compared to the control group . But the index for "Changsha" yellow roosters in tested groups increased by 0 1.46% and 1.2% respectively , compared to the control group . No significant difference was examined to average daily feed intake , too(p>0.05). Feed : gain ratio for l-21d ,22-42d and l-42d , increased by 3.1%, 5.3%, 5.4% respectively for AA hens , and 3.9%, 9.4% and 8.3% respectively for AA roosters ,and 3.8%, 9.0% 7.3% respectively for"Changsha" yellow roosters , compared to the correspanding control groups . But also no significant difference was examined to this index among groups (p>0.05) . Percentage of dressed weight , breast muscle , leg muscle and abdominal fat increased by 0.9% , 1.78% 4.74% -16.79% respectively for AA hens ,and 3.5% 7.58%, -0.26%, -27.86% respectively for AA roosters , and 0, 5.14%), 2.5%, -31.37% for "Changsha" yellow roosters , compared to correspanding control groups . Significant difference was examined to the percentage of abdominal fat among different groups (p<0.05) .TCHO TG, HDL-C, LDL-C, NEFA in serum increased by-11.07%, -28.63%> 10.28%, -0.65%, 15.15% respectively for AA hens , and -7.09%, -9.45%> 28.51%, -3.96%,-1.96% respectively for AA roosters , and -11.22%,-32.91%,0.78%,-1.90%, -13.73% , respectively for "Changsha" yellow roosters , compared to the correspanding control groups . No significant difference was examined to all the indexes among groups (p>0.05) . LPL activity in liver and abdominal fat decreased by 42.85% and 22.88% for AA hens , 26.3% and 28.67% for AA roosters , 37.1% and 17.36% for "Changsha" yellow roosters , compared to the correspanding control groups , But no significant difference was examined among groups to the two indexes (p>0.05) .GH, INS, T3 and T4 in serum increased by 11.23%, 3.7% 15.97% and 40.52% respectively for AA hens ,and 55.44%, 7.25%, 49.25% and 10.57% respectively for AA roosters , and 20%, 48.95% 53.95% and 29.57% respectively for "Changsha" yellow roosters , Compared to the correspanding control groups , No significant difference was examined among groups to the two indexes (p>0.05 ) .Relative expression of PPARa mRNA, PPARr mRNA, LPLmRNA and SCDmRNA in liver increased by 1.39%, -27.27%, -27.23% and -27.65% respectively for AA hens , and 6.43%, -12.5%, -10.04% and -18.83% respectively for AA roosters, and 2.61%,-5.45%,-25.92% and -14.96% respectively for "Changsha" yellow roosters . Relative espression of PPARa mRNA, PPARr mRNA , LPLmRNA and SCDmRNA in abdominal fat increased by 5.35%, -24.87%, -39.87% and -39.59% respectively for AA hens ,and 1.18%, -12.03%, -31.06% and-32.75% respectively for AA roosters ,and 11.56%, -14.8%, -37.56% and -17.29% respectively for "Changsha" yellow roosters ,Compared to the correspanding control groups Significant differences were examined to LPLmRNA expression in abdominal fat and SCDmRNA in liver between AA hens in the tested...
|
PDF Full Text Request