Studies On The Hairiness Character In Pepper (C. Annuum L.)

At present, the research of the hairiness character concerns on cotton, tomato and tea. In order to probe the genetic law, physiologic and biochemical character to the pepper hairiness and quicken the progress of the pepper hairiness character, the genetic studies on pepper hairiness character and molecular mapping of the hairiness gene were carried out.In this study, field identify and micro identify, RAPD(Random Amplified Polymorphic DNA) and BSA(bulked segregant analysis) were used to identify regions associated with the pepper hairiness character in pepper on the F2 segregating generated from the species hairiness and non- hairiness. The following conclusions had been drawn:1.Observation in the pepper hairiness character of hairiness parent(02091), non-hairiness parent(02090), their sons F1 and grand sons F2 populations segregations results showed that the hairiness character was controlled by a single dominant gene and the hairiness character was not a completely dominant genetic character.2. The hairiness dense increased from the bottom to the top in pepper. The hairiness dense decomposition was: the tender stem> stem> leaf rib> leaf contrary facet > leaf obverse facet> leaf margin. The hairiness was composed of 3~10 cells. Then the hairiness length was controlled by the cell number composed the hairiness, and the relative rate of the hairiness length and the cell number was 0.9.3, The results of content of sugar, protein, proline, vitamine C, SOD and the ratio of sugar and protein in pepper indicted that the pepper plant with hairiness had a high content of soluble sugar, the vivid of SOD and the ratio of sugar and protein; while the content of protein, proline and vitamine C was lower.4, The experiment discovered that the hairiness character can decrease the aphid dense and the increment rate, also decrease the aphid to plant damage.5, The optimization RAPD program in pepper was: (1) 94C predenaturation 2min, 92C denaturation for 45s, 36C annealing for 30s, 72C prolong for 75s, 38circles, 72C prolong for 5s, 4C forever. (2)The reaction system was amplified in 25ul reaction volumes each containing 10X PCR buffer 2.5ul, Taq polyrase (2U/ul) 0.5ul, dNTPs (10uM) 0.5ul, primer (8pmol/ul) 1ul, genomic DNA (25ng/ul) 2ul, sterile pure water 18.5ul.6,040,004 and F2 population of 040x004 containing 126 individual plants were used for molecular mapping of hairiness gene by bulked segregant analysis. 280decamer primer with arbitrary sequences were chosen for polymerase chain reaction application. It was found that the polymorphism between the parents could be detected by 15.7% of the primer and one RAPD marker (the sequence of the primer: CGCTTGGCGA) was tightly linked to hairiness gene of 040 with the overcoss walue of 3.97% and genetic distance of 3.98cM.