Analysis On Genetic Diversity Of Chinese Upland Cotton Varieties With Resistance To Fusarium Wilt And Verticillium Wilt

Cotton is an important cash crop in global. Fusarium wilt and Verticillium wilt are two kinds of harms to cotton planting in China. It is confirmed that the most effective and economical method for controlling the two diseases is to select and utilize resistant varieties. Since 1950's we have bred a lot of resistant varieties. In the study, 58 cotton cultivars with resistance to Fusarium wilt and Verticillium wilt which come from different years and provinces .The cultivars was planted in disease nursery of Agricultural University of Hebei to identify disease resistance. In the same time , some field characters were investigated .At last, the genetic diversity of the experiment materials were researched through SSR and AFLP analysis. The results are as follows:1 .The analysis of the yield and fiber quality and of the relation with resistance showed that there was conspicuously different in yield, so the cotton yield can be improved efficiently by planting resistant cultivars. But the fiber quality of the resistant cultivars was not good, some even worse than susceptible cultivars.2. The analysis based on morphological characters showed that the Euclidea distance was among 1.6881-11.1829. Although the range was more extensive the binate distance among the cultivars was small, which indicated that the difference of the morphological characters in one group was small while among groups was large.3. Comparing the result of SSR with that of AFLP, it was showed that the polymorphic information and identification of SSR was higher then AFLP's.With 22 pairs of SSR primers, 254 polymorphic bands were detected, 195 ones with 20 pairs of AFLP primers. 10 cultivars had specific bands related to 10 pairs of SSR primers and 23 cultivars had specific bands related to 15 pairs of AFLP primers. 56 cultivars could be identified by SSR with the most identification was 44 cultivars for BNL1317 primer pairs. By AFLP 50 cultivars could be identified and the most identification was 22 for E35/M59 primer combinations. All experiments materials could be identified by the two methods.4. The Euclidea distance was among 3.6056~ 10.9087 by SSR, among 2.4495~ 7.2111 by AFLP, among 5.5678-12.4900 by the two data, which showed that thegenetic basic among the cultivars is not abundance.5. With the 3 molecular marker methods the materials all could be classified to 5 groups. Each cultivars belongs to different groups but three dengrograms still has some similarity, and the similar degree between SGs and SAGs was higher. Although some cultivars belong to the same group but the distance among the cultivars in a group were very different. All the four cluster could reflect the pedigrees of the cultivars in some degree but is not very anastomose.