Genetic Analysis Of Elite Wheat Line-"Xingzi 9104" Resistance Against Yellow Rust And Powdery Mildew

Stripe rust and powdery mildew are the two most serious wheat diseases which cause heavy loss of the wheat production in the world. Up to now, 32 gene loci for resistance against stripe rust and 30 gene loci for resistance against powdery mildew have been assigned to particular wheat chromosomes in the wold. However, due to the use of resistance genes simplify and the occurrence of new pathogenic virulence of Puccinia striiformis and Blumeria graminis f.sp trtici or ineffective adult resistance, only some of these genes are useable for wheat breeding in China now. These two diseases have been making the serious threaten gradually to the wheat production. Screening and breeding resistance cultivars have been considered to be the most economic, secure and effective measure to control the wheat disease. It is important for the reasonable using of resistance cultivars to know about the constitutes of resistance genes and genetic characters of wheat cultivar and germplasm. The genetic analysis of resistance, gene postulation and AFLP analysis of Xingzi 9104, an elite germplasm, to wheat stripe rust and powdery mildew were conducted in this paper.The results of genetic analysis showed that there were at least one pair of dominant genes to Puccinia striiformis in seedling plants which control the resistance to CY17 of P. striiformis, one pair of dominant independence genes to Puccinia striiformis in adult plants which control the resistance to CY32 of P. striiformis, one pair of dominant genes and one pair of suppressive genes to Blumeria graminis f.sp. trtici in seedling plants and two pairs of dominant complementary genes to Blumeria graminis f.sp. trtici in adult plants which control isolate 031001 of Blumeria graminis f.sp. trtici. The genes YrSK for stripe rust resistance and unknown genes for powdery mildew in seedling stage of Xingzi 9104 were also postulated.The genome DNA of wheat were extracted from the constituted BSA ( bulkedsegregant analysis ) by using of CTAB resistant and susceptible, respectively. Fifty pairs of AFLP primers were used to generate polymorphic bands and one specificDNA fragment was amplified by E01/M10 primer combination in BSA of seedlings resistance gene to stripe rust. The specific DNA polymorphic fragment, a 154 base pair band, was excised and cloned into T-easy vector. Next work is to design the differential primers and transvert it to SCAR. The results indicate that the wheat line Xingzi 9104 may be one of most important materials with resistance in wheat breeding program. It provides a base for achieving diversification of resistance genes in resistance cultivar.