Study On Transmission Of Exogenous Gene Into The Chicken's Early Embryo

To explore the possibility of generation of transgenic chicken, two vectors (p-CMV- β -Gal and p-OV- 3 -Gal) of LacZ gene as report gene were transmitted into the chicken's early embryo by two methods: Microinjection Method and Sperm-mediated Method, then the LacZ gene's integration and expression were monitored. At the same time, the effect of bacteriophage was discussed. The results were as showed:1. After working the egg, a litter of bacteriophage liquor was appended into the egg. Its effect of enhancing the immunity of the embryo and avoiding the embryo dying early was not remarkable. But during the course of the experiment, another effect was discovered: The appended liquor could supply the wet that quickly volatilized in the course of hatching.2. During the course of microinjecting, the structure of the egg was broken. Itresulted in that the growth of the embryo was affected and many embryos died early, the death percentage was 58.07%(367/632). In fact, the vector (p-CMV-β-Gal) could be transmitted into the chicken's early embryo by Microinjection Method, and the percentage of LacZ gene's integration and expression respectively were 21.43%(15/7o)and 9.52 %(4/42)-3. The vector (p-OV- β -Gal) could be transmitted into the chicken's early embryo by sperm-mediated method, the percentage of LacZ gene's integration and expression respectively were 5.91 %(n/,86) and 16.94%(41/242).From the results of the experiments, it could be concluded that two vectors could be transmitted into the chicken's early embryo by microinjection method or sperm-mediated method, and the LacZ gene could be integrated and expressed. It was showed that the two methods could be used for generation of the transgenic chicken.