Function Of ABA To The Increasing Resistence Of Cantharanthus Roseus Seedlings To High Temperature And Its Proteomic Investigation

The unpretreated and ABA pretreated Cantharanthus roseus saplings were exposed to high temperature stress. The physiological indexes correlative to thermo-tolerance were studied in the laboratory, and the expression of total proteins were also detected in the study. Through these we attempted to probe into the mechanism of ABA on Cantharanthus roseus saplings during the heat stress. The result as follows:The ABA pretreated can maintain the activity of SOD and POD, reduce the excessive oxidation of membrane lipid effectively and maintain the stability of membrane structure, also can release the heat injury of chlorophyll a and chlorophyll b.The optimal program for total proteins extraction of Cantharanthus roseus saplings' leaves were selected and the quantification method for proteins from 2-DE were established. The protein expression was analyzed and 32 differentially expressed points were identified through PMF. 10 points were identified better, and 3 points were small heat shock proteins. They were HSP33, HSP18.2, HSP20.0 respectively. Having analyzed the expression of the three points in different gels, we found HSP33 and HSP18.2 could be detected under heat stress without ABA pretreated. while HSP20.0 could be detected under the heat stress with ABA pretreated .The further analyze made it clear that HSP18.2 and HSP20.0 were both constitutively expressed. During the heat stress, the expression of HSP18.2 was up-regulated at the beginning, and decreased markedly with the prolongation of heat stress time. On the contrary, the expression of HSP20.0 rose with the elongation of heat stress time.