Study Of The Antifungal Effect And The Mechanism Of Cupric Complexes Of Glycine To Verticillium Dahliae

By the method of ultraviolet spectroscopy we can determine the complex degree of glycine and cupric sulfate with different quantity of Cu2+, ammonia water mixed and then choose a suitable ratio to compound cupric complexed of glycine . We also try to discover the best consistency of fungicide to restrain pathogenic bacteria through the study of fungicidal action of cupric complexes of glycine, mixed cupric amino-acid complexes and cupric sulfate on Verticillium wilt. The study shows that mixed cupric amino-acid complexes have a good effect to kill Verticillium wilt. And we carry out the fungicidal experiment in the greenhouse and in the field, the results show that mixed cupric amino-acid complexes also have a good effect on cotton Verticillium wilt.Verticillium wilt of cotton plant infected by Verticillium dahbiae Kleb is a kind of plant fungal disease in the vascular bundle which is one of the most serious disease in our country. Cupric complexes of glycine which is new kind antiseptic developed by our laboratory are sprayed on the leaves of cotton plant to prevent and "cure Verticillium wilt of cotton. The inhibiting Verticillium wilt test in lab shows that cupric complexes of glycine could inhibit the grow of the Verticillium wilt in the PDA culture medium. This experiment tends to explain cupric complexes of glycine could induce the resist of cotton plant to the Verticillium wilt through the impact on the activities of several enzymes on cotton leaves made by cupric complexes of glycine. In early stage of exponential phase ,of Verticillium wilt, cupric complexes of glycine was sprayed on the leayes of the healthy and the sick plants which were taken as test materials. The last three functional leaves of each cotton plants which were sprayed 1 -7 hours after, 1 day before and 1 3,5 7,9 days were taken off ,washed with distilledAbstractwater and then were grinded in the phosphate solvents(pH=7) to obtain the enzyme solvents for test. And the activities of the peroxidase(POD), polyphenol oxidase(PPO), catalase(CAT) and phenylalanine aminolyase(PAL) in the test materials are conducted by several corresponding trials. The results show that the activities of POD, PPO, CAT and PAL in the sick plants are higher than the healthy and the above four enzymes activities were hi fluctuation with different degrees. Because the above four enzymes are relative to the resistance of the cotton plant, these results indicate that cupric complexes of glycine may increase the activity of the above four enzymes to reduce the self harm done by over oxidation of lipid membrane; cupric complexes of glycine may also adjust the activity of the oxygen metabolism to induce the resist of the cotton plants and the antifungal activities. In a word, cupric complexes of glycine could increase the activities of POD, PPO, CAT and PAL and reduce the develop of fungal disease of Verticillium wilt of cotton plant.Copper in the root, stem and leaf of cotton which was sprayed by cupric complexes of glycine and water was respectively measured by flame atomic absorption spectrometry. The results show that Cu in the root, stem and leaf of cotton sprayed the cupric complexes of glycine was obviously higher than the cotton sprayed water.