| Chinese chestnut (Castanea mollissima) is an important tree species in China, and nutrition of fruit is abundance. Because its fruit is liked by domestic and international consumers very much, Castanea mollissima has vast development foreground. However, Castanea mollissima cultivation is prior to field seedling propagation now, owing to its taproot characteristic and feeble regeneration capability of root system, lead to injure root seriously when seedlings transplant forestation, revive seedling slowly and fruit late. But the combination of container seedling and mycorrhizal fungi offers a crosscut for improving forestation survival rate of Castanea mollissima.This paper prosecutes container nursery experiments with Castanea mollissima cv. 'Huairou Yanshan' as object, emphasis screening nutrient medium directions, the best inoculation combination of mycorrhiza fungis and inoculation measure. At the same time, the separation, purification, cultivation of Castanea mollissima mycorrhiza fungi are studied, the aim is to bring forward fit series technique measure for Castanea mollissima container seedling production.The results show that nutrient medium confected by surface soil (50%), sand (25%), peatmoss (23%), calcium superphosphate (2%) is ideal prescription for cultivating container seedling of Castanea mollissima , and its primary physical and chemical indexes scopes of nutrient medium are tha total nitrogen is 0.207%~0.335%, rapidly available phosphor is 26.162mg/kg~29.6mg/kg, rapidly available potassium is 167.22mg/kg~ 339.3lmg/kg, organic substance is 3.464 % ~ 8.43 % , rapidly available iron is 5.135mg/kg~6.843mg/kg, rapidly available manganese is 2.58 mg/kg- 13.52 mg/kg, rapidly available copper is 0.436mg/kg ~ 1.133mg/kg, rapidly available zinc is 0.98mg/kg~4.27mg/kg, rapidly available boron is 0.314mg/kg~0.718mg/kg, pH is 5.56-6.89,volume weight is 0.494g~0.820g/cm3, porosity is 72.73 %-77.83 %.The mix inoculation effect of Xerocomus chrysenteron and Cortinarius sublanatus is the best, and solid fungis substance is better.A fungus is separated successfully by sampling from the Castanea mollissima field seedling roots, isolation, purification and cultivation, but its classification position need authenticate further. |