Dehydrin Gene Transformed Petunia Hybrida Showed Strong Resistance To Drought Stress

Water deficiency is a serious limited factor in the agricultural and horticultural development. And drought is the critical factor to affect plant growth and development. Expression of dehydrin increases when plant is under the stess of drought, low tempreture, salt and ABA. This phenomenon indicate that dehydrin could have more important action in improve the tolerant of stress of plant.Dehydrin-like Gene (DBDN) from Boea crassifolia was placed under the control of CaMV35S promoter and inserted into binary vector pCAMBIA3300 for genetic transformation. The transgenic plants of Petunia hybrida were identified by molecular biology procedure and show strong expression of transgene of dehydrin at transcriptional level. The water conservation ability assay and leaf electrolyte leakage determination showed transgenic petunia plants are with strong tolerance to stress condition. Otherwise, put the gene BDN1 under the regulation of T7 promoter and into the prokaryotic expression vector pMXB-10. Express the protein BDN in E. coli ER2566. Collected solubility fusion protein after ultrasonication, cleavage and purification we get the protein BDN1. Use BDN1 immune cony to get antiserum. Potency test of antiserum with ELISA indicate there are antibody of BDN1 in antiserum. The antiserum can be used to test of protein BDN1 expressing in transgenic plant. The work on dehydrin distribution is under further study by immunological method.Master Candidate: Wang JundanMajor: Garden Plant and Ornamental HorticultureSupervisor: Pro. Che Daidi andPro. Lin Zhongping...