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Research On The Characteristics Of Rumen Microbiology Degradation And Acid Production Models In Vitro

Posted on:2005-08-20Degree:MasterType:Thesis
Country:ChinaCandidate:H Z WuFull Text:PDF
GTID:2133360125459132Subject:Animal Nutrition and Feed Science
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The influence of different particle sizes and initial pH buffer in vitro ruem fermentation facility degradation were involved in this paper. The nutrient content based on Van Soet and CNCPS system of seven common southern crude fiber feeds in China and the acid production in rumen artificial degradation after 24 hours fermentation were measured, which were applied to build the regression equations of nutrient content with acid production. Finnally the correlation between the nutrient content and acid production were analysised by connonical statistical method. All the data obtained from this paper can provide a certain amount of data base and theoritical guidelines to improve the degradation ratio by microbiology in the Huanghuai White goat's rumen in vitro fermentation in the south of China, the efficient of natural resource exploiting, the scale of rumen microbiology degradation, and the evaluation of nutrients value in vitro.Experiment 2: Research of different initial pH value on the rumen degradation Buffer liquid pH 4.5,pH5.0, pH5.5, pH6.0,pH6.5 ,pH7.0 ,pH7.5 were designed to evaluate the influence of different initial pH on the rumen degradation. Cellulose was used as the substrate till the end of fermentation. It was found that:Ration of fiber degradation increases with the increase of initial pH value at 4.5 through7.5.Concentration of acetic acid,propionic acid and butyric acid increase with the increase of initial pH among 4.5 to 7.5 at the end of rumen degradation.The pH rang for the most activity of hydroxymethycellulosease is at 5.5 through 5.7.The optimal pH for fiber hydrolysis is between 5.2 and 5.7.The most efficient violate fatty acid production ration is found for the group of initial pH value at 7.0.The microbiology in the rumen can use the acid as subtrate to produce valvic aicd to reduce the effects of end product.Experiment 3: Influence of different fiber particle size on the degradation of rumen degradation Two kinds of particle size cellulose were used as the substrates for the rumen degradation. The results showed: reducing fiber particle size can increase the fiber hydrolysis and the final degradation ration in in vitro rumen fermentation. A high content of acetic acid, propionc acid, butyric acid ,total violate fatty acid and gas production were found in the small fiber particle size group at the end of fermentation.Experiment 4: Measurement of nutrient content of crude fiber feeds and the establishment of the acid production models in rumen fermentation in vitro.Nutrient content based on the Van Soet and CNCPS system of seven kinds of common crude fiber feeds in the south of China and acetic acid,propionic acid, butyric acid after in vitro rumen fermentation were measured in this experiment. The regression model of nutrients content and acid production were built. The results showed: the regression model built on CNCPS nutrients system was superior to evaluate the production of acetic acid and propionic acid which include 90.55%,83.02% of all the information respectively and reached the significant difference level. The regression model built on Van Soet nutrients system was superior to evaluate the production of butyric acid in in vitro rumen fermentation, which included the 81.67% of all the information and reached the significant difference level. The model equations are as follows:Acetic acid:Y1=-1.03X10-3CHO+2.51X10-3CA+6.02X10-3CB1+2.006X10-1CC+1.816 X10-1PA+1.834 X10-1PB1+1.850 X10-1PB2+1.892 X10-1PB3-74.78738(R2=0.9055,P=0.0001)Propionic acid:Y2=-2.8523X10-4CHO-6.7982X10-4CA+1.1502X10-1CC-1.3X10-3CP+1.1576X10-1PA+1.1795 X10-1PB1+1.1668 X10-1PB2+1.1755 X10-1PB3-46.13058 (R2=0.8302,P=0.0013)Butyric acid:Y3=0.30237-1.148X10-4DM-4.06X10-4NDF+9.38X10-5CP-3.1225X10-4CP-6.394X10-5SP-1.15X10-4NPN-3.757X10-4NDFIP+5.9765X10-4ADFIP-4.91X10-3FAT-4.3205 X10-4ASH(R2=0.8167,P=0.0241)It was found from the connonical analysis of nutrients content based on CNCPS system with acid production by in vitro fermentation that the acid produ...
Keywords/Search Tags:rumen microbiology, in vitro fermentation, acid production models
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