The Study Of SeMNPV And The Analysis Of Correlative Genes In Spotoptera Exigua

Spotoptera exigua Multi nucleocapsid Nucleopolyhedrovirus (SeMNPV)can specifically infect the Spotoptera exigua larvae and it has been widely used as a biopesticide in China, But killing slowly is its mainly limitation. The thesis has selected high efficiency viral isolates against Spotoptera exigua Larvae, investigated the potential applications of the cathepsin gene and the superoxide dismutase (sod) gene in the enhancing activities with Spotoptera exigua Multi nucleocapsid Nucleopolyhedrovirus against Spotoptera exigua Larvae. The studies include, for settling a basis of standardization of bio-pesticides, the sequence determination, prokaryotic expression, and bio-function studies of cathepsin gene from Spotoptera exigua Multi nucleocapsid Nucleopolyhedrovirus, the bio-function and enzyme activities of Superoxide Dismutase (SOD) from Spotoptera exigua Multi nucleocapsid Nucleopolyhedrovirus. Results from above studies support our notions that v-CATH (Cathepsin protein of SeMNPV) can be utilized as a bio-pesticide enhancing additive, and Superoxide Dismutase can be used as a light protective agent for bio-pesticides.