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Researches On Cell Wall Glycoprotiein And Sucrose-Metabolizing Of Lycium Barbarum L Under Salt Stress

Posted on:2005-08-17Degree:MasterType:Thesis
Country:ChinaCandidate:J YangFull Text:PDF
GTID:2133360125465569Subject:Crop cultivation and farming
Abstract/Summary:PDF Full Text Request
Several physiological characters of Ningqi No. 1 of lycium barbarum of Ningxia breed are compared under different conditions. Both potted plant and field plant experiments are carried out in order to compare the changes of glycoproteins at the surface of cell, the activities of both glucide and relative enzyme activities under the condition that lyceum barbarum grows at different salt intimidates (0.3%> 0.6% and 0.9%NaCl, respectively). All of these characters are investigated either on cell level, which is through electron microscropy, or on tissue or organic level of both leaves and seeds; At the same time, determination and analysis on the concentration of salts in the soil, together with the concentration of both polysaccharomyces and general sugar of lyceum barbarum are undertook at four production districts in Ningxia, namely, Tongxin, Huinong, Zhongning and Yinchuan. The results are shown as follow:1. The glycoproteins at the cell suface of Lycium barbarum leaves becomes thicker and tight under salt stress. Dinamic changes of the glycoproteins, are orientated by ruthenium and observed through electronmicroscopy under the different salt intimidates. The results show that at the fourthday of treatment, the glycoprotein layer becomes thicker and tighter as the concentration of sodium chloride increases; at the twelfth day, the layer start to getting thicker when 0.3%NaCl is supplemented in the soil and becomes even thicker at 0.6%NaCl stage, however, a high concentration of 0.9%NaCl salt in the soil makes the layer become thinner. In which case, the layer that near the clearance of cell loosens and drops greatly. The droped glycoprotein layer dissociates to the clearance of cells and makes the constructions become blur and some of them ruined.2. The concentration of polysaccharomyces in the leaf and fruit of lyceum barbarum increases as the concentration of sodium chloride increases, and as the salt intimidate time prolonged (P<0.05) . The concentration of polysacchromyces in the fuit of lyceum barbarum of both 0.3% and 0.6%NaCl groups increase continuously, on the contrary, it decreases in the 0.9%NaCl group.3. The solubility sugar increases significantly (P<0.05) in the leaf of lyceum barbarum, while the solubility sugar in the seeds of lycium barbarum exhibits trend to decrease, whereas the starch decreases significantly (P<0.05) . The concentration of cane sugar of both leaf and fuit tend to increase, while the reducing sugar of both leaf and fuit keeps a trend to decrease.4. Along with the increases of concentration of sodium chloride,the activities of relative enzymes in the leaf of lyceum barbarum change significantly. Along with the increases of concentration of sodium chloride, the activity of neutral invertase in the leaf and fruitdecreases significantly(P<0.05); The activity of acid invertase in both leaf and fruit trend to decrease, whereas 0.6%NaCl shows little influence on the activity of acid invertase of leaf and decreases the activity of acid invertase of fruit. 0.6%NaCl decreases the enzyme activity of sucrose synthetase in the leaf, but has no effect on the activity of Sucrose phosphate synthase The activity of SS decreases first, and then increases, while SPS shows little change when 0.6%NaCl is added. 0.9%NaCl can reduce the activity of both AI and SPS significangly (P<0.05) . 0.3% NaCl activates SPS, 0.6%NaCl shows little influence on it, while 0.9% reduces its activity. The activity of SS can be infected either to decrease hi 0.6% NaCl or to increase in 0.9% NaCl group.5. The salts in the soil of four production districts in Ningxia are analyzed. The results demonstrated that the salts in the soil of Tongxin and Huinong are comparatively higher and therefore, the concentrations of Polysaccharomyces are also higher if compared with other two districts. One of the main construction ions, Na+ and Cl" have significant relativity with Polysaccharomyces in the seeds of lyceum barbarum, the coefficient of correlations are 0.504 and 0.54, respectively. The correlation between polysac...
Keywords/Search Tags:Salt stress, Lycium barbarum L, glycoprotiein, Sugar-Metabolism
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