The Selecting And The Studying Of Effective Components Absorbed Into Blood From Huang Lian Jie Du Tang

Objective: In research on the pharmacological activities of Huang Lion Jie Du Tang (HLJDT)--a traditional Chinese compound preparation-the dose were feeded to rats and abstract absorbed components of HLJDT from the serum of the rats under the direction of absorbed theory. By doing that we prepared a great deal of samples containing baicalein,baicalen ,wogonoside , wogonin and geniposide and the total of them. During the bioactivity experiment of inhibit bacteria ,anti-inflammatory ,regulating the immune under the same conditions ,we studied the bioactivity effects of the prepared dose to confirm its pharmacological activities ,select and analysis the activities of the components ,discuss the application of the HLJDT in clinical course and provide the material for the further pharmacological study .Contents and methods 'we prepare and analysis the samples according to the absorbed theory .To test the inhibit effects about 8 strains bacteria ,we added the samples to the plates cultured with bacteria,to study the immunoregulating effects of the samples ,we feeded samples to mouse and observed the weight and the structures of its Thymus Gland and Spleen ,the macrophage cell and T lymphocyte's function affected by baicalein,baicalen ,wogonoside , wogonin and geniposide and the total of them. In studying the anti-inflammatory mechanism, we stimulateTHP-1 -a model for test the ability of produce cytokines, with LPS 10 u g /ml+PMA 200nmol/ml, the production of the cytokine reach a peak . The effects of the samples regulating TNFa and IL-1B produced by THP-1 were studied by enzyme-linked immunosorbent assay (ELISA).Results : In inhibiting bacteria experiments ,all the samples suppressed the multiplication of the bacteria in different degrees and the sample contain all components makes a significant suppressed effect . The results of observing Thymus Gland and Spleen of mouse showed the samples did not affect the developing of Thymus Gland and Spleen weight, but suppressing the multiplication of the lymphocytes in it except baicalein .the samples increasing the swallow ability of the Macrophage cell and all the samples except baicalein suppressed the T lymphocyte's developing course .All samples inhibited the production of TNFa and IL-1B by THP-1 was stimulated LPS and phorbo-lmyristate acetate (PMA),and it is that significantly inhibit the production of TNFa and IL-1B.Conclusion : The effective components absorbed into blood in HLJDT can inhibit bacteria ,perform anti-inflammatory ,regulat immune in different degrees, geniposide, baicalein,baicalen ,wogonoside and wogonin are bioactivity components in HLJDT.