Study And Preparation Of Herbal Compound Against Cow's Mastitis

According to "Veterinary Pharmacopoeia of PRC. " and a great deal of references on Chinese herbs, nine kinds of herbs usually used to treat cow' s mastitis were chosen, which are Flos Lonicerae, Herba Taraxaci, Radix Isatidis, Pericarpium Citri Reticulatae, Fructus Forsythiae, Spina Gleditsiae, Radix Scutellariae, Hibiscus Mutabilis L. and Herba Houttuyniae. Their optimum extractions were investigated by orthogonal test and antibiotic activity of herbal extracts determined. The results showed that ethanol extracts of Flos Lonicerae, Spina Gleditsiae, Radix Scutellariae, Pericarpium Citri Reticulatae and Hibiscus Mutasilia can inhibit Staphylococcus aureus and Escherichia coli from cows' mastitis and those of Herba Houttuyniae and Fructus Forsythiae only can inhibit Staphylococcus aureus. Extracts with activity against Escherichia coli are ones extracted with ethanol from Herba Taraxaci and Radix Isatidis and with water from Spina Gleditsiae, In the light of the above-mentioned, it can be concluded that pathogens are different to all kinds of extracts in sensitivity.Under the direction of Chinese Medicine, compounds 1 ?10 were prepared by optionally adding herbal extracts to ethanol extracts of Flos Lonicerae and Herba Taraxaci. Their inhibition to Staphylococcus aureus and Escherichia coli was determined, and compared with extracts of traditional composition. Based on the results, compounds 4, 5, 7 and 9 that have stronger antibiotic activity were sorted out, whose components are as follows:4: Flos Lonicerae: Herba Taraxac: Fructus Forsythiae: Pericarpium Citri Reticulatae 2:3:2:15: Flos Lonicerae: Herba Taraxaci: Spina Gleditsiae: Hibiscus Mutasilia= 2:3:2:2,7: Herba Taraxaci: Spina Gleditsiae: Fructus Forsythiae: Pericarpium Citri Reticulatae=3:4:2:1,9: Flos Lonicerae: Spina Gleditsiae: Herba Houttuyniae: Hibiscus Mutasilia=3:4:2:2.