Study On Enzyme-linked Immunosorbent Assay For Degradation Product Of Deltamethrin

The object of this research is to establish the enzyme-linked immunosorbent assay(ELISA) for the residue of deltamethrin (DM) . Based on the structure of deltamethrin acid (DA), Two haptens were synthesized andtheir structure were identified. Two hapten were linked with one of carriers (four proteinsand one MAP), respectively. All the conjugates were identified and the haptens to carrierratio was estimated with ultraviolet spectrophotometry. Conjugates HⅠ-BSA,HⅡ-BSA were used to immunize mouse to prepare polyclonalantibodies (PcAbs) for deltamethrin . The titers of two kinds of antisera were 6.4×104 and3.2×104 determined by indirect ELISA, respectively. Two PcAbs were used to establish indirect ELISA for ramification of degradationproducts of DM, respectively. The fortified recoveries for ramification of degradationproducts of DM in Water samples were determined. For indirect ELISA used HⅠ-BSA-2, the IC50 of HⅠand HⅡare 54.5 ng/mL and26.9 ng/mL, respectively. The cross-reactivity of PcAb with Pyrethroids and DA are alllower than 0.1%. When the accession concentration of HⅠand HⅡ in water samples are50 ng/mL and 30 ng/mL, the recoveries are 75.64～90.45% and 87.30～104.32% byindirect ELISA, respectively. For indirect ELISA used HⅡ-BSA-3, the IC50 of HⅠ和 HⅡare 63.2 ng/mL 和 5.56 2新疆农业大学硕士学位论文 英文摘要ng/mL, respectively. The cross-reactivity of PcAb with Pyrethroids and DA are all lowerthan 0.1%. When the accession concentration of HⅠand HⅡin water samples are 75ng/mL and 5 ng/mL, the recoveries 73.4～88.7% and 80.8～105.4% by indirect ELISA,respectively. The research of PcAbs for deltamethrin is reported first time in China. The establishedindirect ELISA of HⅠ-BSA-2 and HⅡ-BSA-3 is sensitive and specific for HⅠand HⅡ,so that it can be used to detect ramification of degradation products of DM .