Studies On Some Immunity Indexes In The Blood Serum Of Fenneropenaeus Chinensis And The Methods Of Purification Of β-1.3-glucan Binding Protein

Fenneropenaeus chinensis a very important commercial animal on Chinese marine-culture, however, the shrimp industry has always been affected by infectious diseases, mainly of bacterial and viral etiology.The research on the immunity mechanism of shrimp become an urgent and basic need. The immunity indexes of cellular and humoral immune system were investigated to find the most effective one for the selection of immunostimulants .To explain the causes of the immune response about the shrimp, several methods were tried to purified the beta-1,3-glucan binding protein.. Several changes of protein activities could be used to determine the health of the shrimps, and the activities of phosphatase and superoside dismutase were more often used in the experiment of the humoral immunity indexes.After the injection of lipopolysaccharide and the Vibrio anguillarum individually and the changes of the temperature and the salinity , the activities of acid phosphatase(ACP), superoside dismutase(SOD) and the content of hemocyanin were determined. The method of determining the content of hemocyanin was first used in Fenneropenaeus chinensis The results showed that the activites of ACP enhanced after the injection of Vibrio anguillarum , however the activites of SOD changed little through the same procedure. After the shrimp was stimulated by the lipopolysaccharide(10μg/g), the activities of ACP and SOD enchanced slightly. To find the causes of immunity responses of the Chinese shrimp, three methods were tried to purified the beta-1,3-glucan binding protein(βGBP).The result showed that the βGBP 100 appeared clear in the eluted proteins from the immobilized glucan column. Some other faint proteins were found at the same lane and the main functions of these proteins should be deeper investigated .This study shows that the immobilized glucan method is a better way to find the βGBP 100 and other deeper methods should be more used to separate the βGBP 100 from the other proteins which seem also to bind the immobilized glucan weakly.