Preliminary Study On Enhancing Immunity Of Neonatal Giant Panda (Ailuropoda Melanoleuca)

The objective of this study was to do the preliminary studies on enhancing immunity of neonatal giant panda. Three experiments were carried out. Experiment 1 assayed three formulas of artifical milk for giant panda cub and colostrum of giant panda, black bear, goat. Experiment 2 detected the newborn giant panda cub's intestine flora. The aim of experiment 3 was to do preliminary studies of confecting artifical colostrum for neonatal giant panda. Results as follows:1 Four items of each testee milk were tested, crude protein, ether extract, lactose and leral elements, there were 7 kinds of mineral elements were tested. By analysed theresults, author chose the formula from Chengdu research base of giant panda breedingas the most suitable one for panda cub. The formula is goat colostrum : pandamilk(from Janpan): deion water =15:5 :30 (by weight)2, The dominant, detectable bacteria of newborn giant panda cub's intestine flora were Escheia coli and streptococcus faaecalis by panda cub was 35-day-old. When the cub was 25-day-old lactobacillus was detected in feces, lactobacillus was low percentage of the flora. Till the cub was 2-month-old no Bifidobacterium was detected. The results showed the intestine flora of newborn giant panda cub was unique.3 The results of assaying total protein and the proportions of different protein contents of newborn giant panda cub serum showed that panda cub have low immunity. Theresults showed unitil panda cub was 19 weeks old, the highest Gamma Globin concentration in serum was only 1.9g/l, the concentration was adult's 22.62%, and Gamma Globin proportion was 4.1% total serum protein, the same item of adult panda was 12.09%. One cub died 13-day-old , the cub's serum was assayed, the Gamma Globin concentration in serum was only 0.4g/l, the concentration was adult's 4.76%, Gamma Globin proportion was 4.1% total serum protein. Based on the results author recommended a formula, the formula was used to calculate the volume of adults panda serum that fed to newborn giant panda cub as colostrums supplement, the formula as follows:The volume of serum fed to the cub=7.5ml/100g body weightxcub's body weight X 50% X 6mg/ml 50% 8.4mg/ml4 Healthy adult giant panda's serum was lyophilized. After the trial of microbe culture and fed to sucking mice, the lyophilized serum was testified safety, will not make the testee animal diarrhoea and growth hypogenesis.