Pilot Study Of Receptor And Structural Proteins Of WSSV

Cell plasma membranes were obtained from Litopenaeus vannamei gill by centrifugation. WSSV were obtained from L. vannamei that infected shrimp white spot syndrome by centrifugation in sucrose gradient. Binding between WSSV and cell plasma membranes was investigated by an ELISA-like solid phase binding assay. The binding was saturable and special in this assay, which accord with the binding characteristic between virus and receptor.WSSV receptor on the cell membrane was studied by virus overlay protein blot assay (VOPBA). WSSV receptor attachment proteins on WSSV were studied by a method like VOPBA. The receptor and the attachment proteins were sensitive to temperature, pH, salinity, concentration, and time of reaction, thus this two experiments didn't get perfect results. But it gave us some primary knowledge about WSSV receptor and WSSV receptor attachment proteins.It was proved that the complete genome sequence of WSSV contains 292,967 nucleotides encompassing 184 major open reading frames (ORFs). The molecular weight of the proteins of WSSV encoded by those ORFs will have a large span from 6 KD to 664 KD in theory. Many articles about structural proteins of WSSV were reported since 2000, but no article about high molecular weight structural proteins of WSSV was reported up to now. In this article 13 high molecular weight structural proteins of WSSV were first found out by SDS-PAGE, their molecular weight and content were accounted. VP164, VP184 and VP220 were selected to determine the amino acid sequence by MALDI-TOF-MS/MS. The ORFs that encoding VP164, VP184 and VP220 were located by search database. This is a pioneer in the study about WSSV.