A Study On Organ Culture And Protoplast Culture Of Kenaf

As an important fiber crop, many potential applications of kenaf are being identified and developed in 21 century, especially in developed countries such as America, Japan, and France. So make species innovation for kenaf is necessary. It's organ culture and protoplast culture is important basic technique of rapid clonal propagation, selection of imitative plants, somatic hybridization and genetic transformation. In the study, hypocotyl, internodle stem, cotyledon, leaf, axillary bud and protoplast were cultured. The main results are as follows:1 .By studys on effects of different hormone combinations on the induction of calli from different explants, the result indicated that: 2,4-D at lower density (0.05 mg/L, 0.1 mg/L), the white hard callus initiate from epidermis and cortex of hypocotyl; 2,4-D at higher density(1.0 mg/L, 2.0 mg/L), the yellow loose callus initiate from vascular cambium of hypocotyls. The two kinds of callus issue from hypocotyls and internodle stem sections have high callus ratio and good growth. The optimal hormone combination on the induction of calli from cotyledon and leaf is (1.0-2.0) mg/L 2,4-D+(0.1-1.0) mg/L 6-BA.2.The cotyledon and leaf can be directly induced adventitious shoots on MS with 3.5 mg/L TDZ and 0.1 mg/L NAA. The variety 722 can obtain highest induction ratio-13 shoots/explant at the same condition. Adventitious shoot was not obtained from 917.3.Nodle stem sections placed on MS medium supplemented with 0.1 mg/L 6-BA for axillary bud initiation can obtain normal strong shoots;on MS with 0.2 mg/L TDZ can induced multiple shoots, Shoots elongated and rooted within 2 weeks after subculture on MS with 0.1 mg/L NAA.4.Studied effets of LAA, NAA, 6-BA on hypocotyls rooting. A systematic histologucal study indicated direct adventitious roots originated from vascular cambium in hypocotyls.5.The highest protoplast yield and best activity was obtained from solution of Cellulose R-10 0.5%, Pectinase 0.5% and 0.45M Mannitol with the treatment of 12h for the 3d hypocotyls in the dark.6.The results of different mannitol concentration, culture media, and culture methods on the division and growth of protoplast had been studied. Under the condition of 5.0x104/ml and modified KM8P, 0.5mg/L 2,4-D and 0.5mg/L 6-BA,droplet culture was the best and beneficial of the division of protoplast and the growth of calli.