The Isolation Of The APETALA3 CDNA In Brassica Napus And The Morphology Obervation, The Soluble Protein Analysis In Apetalous Oilseed Rape

By using RT-PCR with primers designed according to the APETALA3 cDNA sequence of Brassica napus, two APETALA3 cDNA fragments were obtained. Sequence analysis showed that the two cDNA fragments were 654bp and 675bp, respectively, and shared high identity with those of B. napus, B. uleracea and Arabidopsis thaliana. They have highly conserved MADS-domain but remarkably differences in the C-terminal region. The result showed that there are at least two APETALA3 genes regulating themselves or downstream target-genes' expression during the early floral development in B. napus. Although they play similar role on protein and protein interaction, they maybe have different transcriptional capacity. Hence, we conclude that they co-regulate floral development processes by different ways. In addition, owing to multlicopy phenomena, floral development in brassica napus become more complex.morphology research shows floral buttresses arise on the flank of the apical meristm in Brassica napus and Scu apetalous mutant, and have noremarkable different. After sepal primordial appear, the abaxial sepal primordium begin to overlie the dome-shaped flower primordium, at the same time stamens primordium begein to development, however petal primordium does not appear. When floral organ size is at the scale from 1mm to 1.5mm. Barely visible are the petal primorida that arises between the sepals and close to their base in wild Brassica napus, but petal primorida does not appear in Scu apetalous mutant.By means of vertical slab polyacrylamide gel electrophoresis, the soluable proteins and isoenzymes of superoxidase in Brassica napus and Scu apetalous mutant were analyzed. Results showed that SDS-PAGE patterns of soluable proteins between B.napus and apetalous mutant were of no significant difference, but isoenzyme patterns of superoxidase have significant difference during early floral development. At the stage of floral organ mature, their patterns of soluable proteins exists magnificent difference, and those of superoxidase were extraordinarily similar. We suggest that apetalous phenotype directly affected the floral development in B.napus, and then lead to a series of reaction varies of physiology and biochemistry.